| RRC ID |
65861
|
| 著者 |
Sato K, Takei M, Iyota R, Muraoka Y, Nagashima M, Yoshimura Y.
|
| タイトル |
Indomethacin inhibits melanogenesis via down-regulation of Mitf mRNA transcription.
|
| ジャーナル |
Biosci Biotechnol Biochem
|
| Abstract |
Non-steroidal anti-inflammatory drugs (NSAIDs) exhibit several divergent biological effects. In this study, we investigated the effect of indomethacin on melanin synthesis using B16F1 melanoma cells. Indomethacin inhibited α-melanocyte stimulating hormone (α-MSH)-enhanced melanin synthesis in a dose-dependent manner. Western blotting analysis revealed that indomethacin significantly suppressed tyrosinase and Mitf protein levels. In a luciferase reporter assay, we found that indomethacin reduced tyrosinase promoter activity. Moreover, real-time RT-PCR analysis showed that indomethacin lowered mRNA levels of melanogenic genes, including Mitf. Together, our findings indicate that indomethacin inhibits melanogenesis via the suppression of Mitf transcription.
|
| 巻・号 |
81(12)
|
| ページ |
2307-2313
|
| 公開日 |
2017-12-1
|
| DOI |
10.1080/09168451.2017.1394812
|
| PMID |
29090638
|
| MeSH |
Animals
Cell Line, Tumor
Cell Proliferation / drug effects
Down-Regulation / drug effects*
Indomethacin / pharmacology*
Intracellular Space / drug effects
Intracellular Space / metabolism
Melanins / biosynthesis*
Melanoma, Experimental / pathology
Mice
Microphthalmia-Associated Transcription Factor / genetics*
Monophenol Monooxygenase / genetics
Monophenol Monooxygenase / metabolism
Promoter Regions, Genetic / genetics
RNA, Messenger / genetics
RNA, Messenger / metabolism
Transcription, Genetic / drug effects
|
| IF |
1.516
|
| リソース情報 |
| ヒト・動物細胞 |
B16F10(RCB2630) |
