RRC ID 6587
Author Taira M, Nezu T, Sasaki M, Kimura S, Kagiya T, Harada H, Narushima T, Araki Y.
Title Gene expression analyses of human macrophage phagocytizing sub-micro titanium particles by allergy DNA chip (Genopal).
Journal Biomed Mater Eng
Abstract The purpose of this study was to examine gene expressions of macrophage phagocytizing sub-micro Ti particles by a DNA chip. Human monocytic cell line THP-1 was differentiated into macrophages by culturing for two days in medium supplemented with 200 nM phorbol ester (PMA). The macrophages were then cultured in four media: medium without PMA (control); medium with suspended sub-micro Ti particles (0.5 wt%); medium with 1.0 microg/ml lipopolysaccharide (LPS); and medium with LPS and Ti particles. After 6 hours' culture, total RNA were extracted and gene expressions were evaluated by DNA allergy chip with 205 allergy and inflammation related gene spots. We found that phagocytosis of sub-micro Ti particles and LPS independently and synergistically up-regulated 17 inflammation-related genes more than two-fold. The extensive expressions of four genes (CCL1, IL1B, IL6 and IL8) were further confirmed by real-time quantitative PCR. It turned out that dual stimulation of LPS and Ti particles most up-regulated three genes (IL1B, IL6 and IL8), followed by LPS while Ti particles moderately but least increased, suggesting that phagocytosis of sub-micro Ti particles induces moderate inflammation with its degree less than LPS, but phagocytosis of sub-micro Ti particles has the potential to worsen inflammation caused by LPS-stimulated macrophages.
Volume 19(1)
Pages 63-70
Published 2009
DOI 10.3233/BME-2009-0564
PII G8433R853V624321
PMID 19458447
MeSH Biological Assay / methods* Cells, Cultured Cytokines / analysis Cytokines / metabolism* Gene Expression Profiling / methods* Humans Macrophages / physiology* Nanoparticles* Oligonucleotide Array Sequence Analysis / methods* Phagocytosis / physiology* Titanium*
IF 0.993
Times Cited 10
Human and Animal Cells