| RRC ID |
65879
|
| 著者 |
Hadisaputri YE, Miyazaki T, Yokobori T, Sohda M, Sakai M, Ozawa D, Hara K, Honjo H, Kumakura Y, Kuwano H.
|
| タイトル |
TNFAIP3 overexpression is an independent factor for poor survival in esophageal squamous cell carcinoma.
|
| ジャーナル |
Int J Oncol
|
| Abstract |
Tumor necrosis factor α induced protein 3 (TNFAIP3) is a protein that is induced by TNF-mediated NF-κB activation and has a dual function in regulating NF-κB. TNFAIP3 is associated with inflammatory carcinogenesis in many cancer types. However, the clinical significance of TNFAIP3 expression and function in esophageal squamous cell carcinoma (ESCC) has not yet been reported. We examined 149 ESCC tissue specimens to determine the clinical significance of TNFAIP3 by immunohistochemistry. Western blot analyses were used to detect TNFAIP3 expression in TE-1, TE-8, TE-15 and KYSE-70 ESCC cells and in Het-1A, a non-cancerous esophageal cell line. TNFAIP3 protein knockdown was conducted using small-interfering RNA to investigate its impact on cell proliferation, migration and invasion. Significant correlations between TNFAIP3 expression and differentiation (P=0.04) among clinicopathological characteristics of ESCC patients were demonstrated, and high TNFAIP3 expression was associated with poor survival (P=0.02). Moreover, multivariate analysis result showed that high TNFAIP3 expression was an independent factor for poor survival (P=0.04). In vitro analysis showed high expression of TNFAIP3 protein in TE-15 cells and low expression in Het-1A cells. Furthermore, the proliferation, migration and invasion of TE-15 cells after TNFAIP3 suppression by siRNA were significantly reduced. These findings suggest that TNFAIP3 protein may be an independent prognostic marker for poor survival, and a promising target for ESCC therapy.
|
| 巻・号 |
50(3)
|
| ページ |
1002-1010
|
| 公開日 |
2017-3-1
|
| DOI |
10.3892/ijo.2017.3869
|
| PMID |
28197630
|
| MeSH |
Adult
Aged
Aged, 80 and over
Carcinoma, Squamous Cell / mortality
Carcinoma, Squamous Cell / pathology*
Cell Line, Tumor
Cell Movement / genetics*
Cell Proliferation / genetics
Esophageal Neoplasms / mortality
Esophageal Neoplasms / pathology*
Esophageal Squamous Cell Carcinoma
Female
Humans
Immunohistochemistry
Male
Middle Aged
NF-kappa B / metabolism
Neoplasm Invasiveness / genetics*
Prognosis
RNA Interference
RNA, Small Interfering / genetics
Tumor Necrosis Factor alpha-Induced Protein 3 / genetics*
Tumor Necrosis Factor alpha-Induced Protein 3 / metabolism*
|
| IF |
3.899
|
| リソース情報 |
| ヒト・動物細胞 |
TE-1(RCB1894)
TE-8(RCB2098)
TE-15(RCB1951) |
