RRC ID |
6587
|
著者 |
Taira M, Nezu T, Sasaki M, Kimura S, Kagiya T, Harada H, Narushima T, Araki Y.
|
タイトル |
Gene expression analyses of human macrophage phagocytizing sub-micro titanium particles by allergy DNA chip (Genopal).
|
ジャーナル |
Biomed Mater Eng
|
Abstract |
The purpose of this study was to examine gene expressions of macrophage phagocytizing sub-micro Ti particles by a DNA chip. Human monocytic cell line THP-1 was differentiated into macrophages by culturing for two days in medium supplemented with 200 nM phorbol ester (PMA). The macrophages were then cultured in four media: medium without PMA (control); medium with suspended sub-micro Ti particles (0.5 wt%); medium with 1.0 microg/ml lipopolysaccharide (LPS); and medium with LPS and Ti particles. After 6 hours' culture, total RNA were extracted and gene expressions were evaluated by DNA allergy chip with 205 allergy and inflammation related gene spots. We found that phagocytosis of sub-micro Ti particles and LPS independently and synergistically up-regulated 17 inflammation-related genes more than two-fold. The extensive expressions of four genes (CCL1, IL1B, IL6 and IL8) were further confirmed by real-time quantitative PCR. It turned out that dual stimulation of LPS and Ti particles most up-regulated three genes (IL1B, IL6 and IL8), followed by LPS while Ti particles moderately but least increased, suggesting that phagocytosis of sub-micro Ti particles induces moderate inflammation with its degree less than LPS, but phagocytosis of sub-micro Ti particles has the potential to worsen inflammation caused by LPS-stimulated macrophages.
|
巻・号 |
19(1)
|
ページ |
63-70
|
公開日 |
2009-1-1
|
DOI |
10.3233/BME-2009-0564
|
PII |
G8433R853V624321
|
PMID |
19458447
|
MeSH |
Biological Assay / methods*
Cells, Cultured
Cytokines / analysis
Cytokines / metabolism*
Gene Expression Profiling / methods*
Humans
Macrophages / physiology*
Nanoparticles*
Oligonucleotide Array Sequence Analysis / methods*
Phagocytosis / physiology*
Titanium*
|
IF |
1.243
|
引用数 |
10
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
リソース情報 |
ヒト・動物細胞 |
THP-1(RCB1189) |