RRC ID 65893
著者 Takeuchi Y, Narumi R, Akiyama R, Vitiello E, Shirai T, Tanimura N, Kuromiya K, Ishikawa S, Kajita M, Tada M, Haraoka Y, Akieda Y, Ishitani T, Fujioka Y, Ohba Y, Yamada S, Hosokawa Y, Toyama Y, Matsui T, Fujita Y.
タイトル Calcium Wave Promotes Cell Extrusion.
ジャーナル Curr Biol
Abstract When oncogenic transformation or apoptosis occurs within epithelia, the harmful or dead cells are apically extruded from tissues to maintain epithelial homeostasis. However, the underlying molecular mechanism still remains elusive. In this study, we first show, using mammalian cultured epithelial cells and zebrafish embryos, that prior to apical extrusion of RasV12-transformed cells, calcium wave occurs from the transformed cell and propagates across the surrounding cells. The calcium wave then triggers and facilitates the process of extrusion. IP3 receptor, gap junction, and mechanosensitive calcium channel TRPC1 are involved in calcium wave. Calcium wave induces the polarized movement of the surrounding cells toward the extruding transformed cells. Furthermore, calcium wave facilitates apical extrusion, at least partly, by inducing actin rearrangement in the surrounding cells. Moreover, comparable calcium propagation also promotes apical extrusion of apoptotic cells. Thus, calcium wave is an evolutionarily conserved, general regulatory mechanism of cell extrusion.
巻・号 30(4)
ページ 670-681.e6
公開日 2020-2-24
DOI 10.1016/j.cub.2019.11.089
PII S0960-9822(19)31594-5
PMID 32004455
MeSH Animals Calcium Signaling / physiology* Cell Transformation, Neoplastic / metabolism* Dogs Embryo, Nonmammalian Madin Darby Canine Kidney Cells Zebrafish
IF 9.601
リソース情報
ゼブラフィッシュ Tg(krt4:Gal4) zf422