RRC ID 65901
Author Trolet A, Baldrich P, Criqui MC, Dubois M, Clavel M, Meyers BC, Genschik P.
Title Cell Cycle-Dependent Regulation and Function of ARGONAUTE1 in Plants.
Journal Plant Cell
Abstract Regulated gene expression is key to the orchestrated progression of the cell cycle. Many genes are expressed at specific points in the cell cycle, including important cell cycle regulators, plus factors involved in signal transduction, hormonal regulation, and metabolic control. We demonstrate that post-embryonic depletion of Arabidopsis (Arabidopsis thaliana) ARGONAUTE1 (AGO1), the main effector of plant microRNAs (miRNAs), impairs cell division in the root meristem. We utilized the highly synchronizable tobacco (Nicotiana tabacum) Bright yellow 2 (BY2) cell suspension to analyze mRNA, small RNAs, and mRNA cleavage products of synchronized BY2 cells at S, G2, M, and G1 phases of the cell cycle. This revealed that in plants, only a few miRNAs show differential accumulation during the cell cycle, and miRNA-target pairs were only identified for a small proportion of the more than 13,000 differentially expressed genes during the cell cycle. However, this unique set of miRNA-target pairs could be key to attenuate the expression of several transcription factors and disease resistance genes. We also demonstrate that AGO1 binds to a set of 19-nucleotide, tRNA-derived fragments during the cell cycle progression.
Volume 31(8)
Pages 1734-1750
Published 2019-8-1
DOI 10.1105/tpc.19.00069
PII tpc.19.00069
PMID 31189739
PMC PMC6713298
MeSH Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis / physiology* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Argonaute Proteins / genetics Argonaute Proteins / metabolism* Cell Cycle / genetics Cell Cycle / physiology* Gene Expression Regulation, Plant / genetics Gene Expression Regulation, Plant / physiology* MicroRNAs / genetics MicroRNAs / metabolism RNA, Messenger / genetics RNA, Messenger / metabolism
IF 9.618
Arabidopsis / Cultured plant cells, genes rpc00001