論文 - 詳細
| RRC ID | 65902 |
|---|---|
| 著者 | Vanholme R, Sundin L, Seetso KC, Kim H, Liu X, Li J, De Meester B, Hoengenaert L, Goeminne G, Morreel K, Haustraete J, Tsai HH, Schmidt W, Vanholme B, Ralph J, Boerjan W. |
| タイトル | COSY catalyses trans-cis isomerization and lactonization in the biosynthesis of coumarins. |
| ジャーナル | Nat Plants |
| Abstract |
Coumarins, also known as 1,2-benzopyrones, comprise a large class of secondary metabolites that are ubiquitously found throughout the plant kingdom. In many plant species, coumarins are particularly important for iron acquisition and plant defence. Here, we show that COUMARIN SYNTHASE (COSY) is a key enzyme in the biosynthesis of coumarins. Arabidopsis thaliana cosy mutants have strongly reduced levels of coumarin and accumulate o-hydroxyphenylpropanoids instead. Accordingly, cosy mutants have reduced iron content and show growth defects when grown under conditions in which there is a limited availability of iron. Recombinant COSY is able to produce umbelliferone, esculetin and scopoletin from their respective o-hydroxycinnamoyl-CoA thioesters by two reaction steps-a trans-cis isomerization followed by a lactonization. This conversion happens partially spontaneously and is catalysed by light, which explains why the need for an enzyme for this conversion has been overlooked. The combined results show that COSY has an essential function in the biosynthesis of coumarins in organs that are shielded from light, such as roots. These findings provide routes to improving coumarin production in crops or by microbial fermentation. |
| 巻・号 | 5(10) |
| ページ | 1066-1075 |
| 公開日 | 2019-10-1 |
| DOI | 10.1038/s41477-019-0510-0 |
| PII | 10.1038/s41477-019-0510-0 |
| PMID | 31501530 |
| MeSH | Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Catalysis Coumarins / metabolism* Glycosides / biosynthesis Isomerism Mutation Plant Roots / metabolism Pregnenolone / analogs & derivatives Pregnenolone / biosynthesis Scopoletin / metabolism Umbelliferones / biosynthesis |
| IF | 13.256 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | pst19616 pst16357 |