論文 - 詳細
| RRC ID | 65906 |
|---|---|
| 著者 | Takahashi K, Ohta T. |
| タイトル | Membrane translocation of transient receptor potential ankyrin 1 induced by inflammatory cytokines in lung cancer cells. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Transient receptor potential ankyrin 1 (TRPA1) is known as one of the nociceptors expressed in sensory neurons. It also plays a role in non-neural cells in inflammatory sites. However, the regulatory mechanisms for the reactivity of TRPA1 in these cells under inflammatory conditions are not clear. To clarify these mechanisms, we examined the effects of inflammatory cytokines (interleukin [IL]-1α, IL-1β and tumor necrosis factor α [TNFα]) on TRPA1 reactivity and expression in the endogenously TRPA1-expressing lung tumor cell line A549. Treatment with IL-1α, but not IL-1β or TNFα, increased the number of cells responding to allyl isothiocyanate, a TRPA1 agonist, in a dose- and time-dependent manner. The IL-1α-induced increase of TRPA1 responsiveness was inhibited by an extracellular-regulated kinase (Erk) inhibitor (PD98059) but not by inhibitors of c-Jun kinase, p38 mitogen-activated protein kinase or phosphatidylinositol-3 kinase. Phosphorylation of Erk gradually increased at 24 h after its transient induction in cells treated with IL-1α. IL-1α increased the TRPA1 levels on biotinylated cell surface proteins. These results suggest that IL-1α enhances the translocation of TRPA1 to the plasma membrane via the activation of Erk in A549. TRPA1 may have a pathophysiological role in non-neural lung cells under inflammatory conditions. |
| 巻・号 | 490(3) |
| ページ | 587-593 |
| 公開日 | 2017-8-26 |
| DOI | 10.1016/j.bbrc.2017.06.082 |
| PII | S0006-291X(17)31213-5 |
| PMID | 28629997 |
| MeSH | A549 Cells Calcium Channels / immunology* Cell Membrane / immunology Cell Membrane / metabolism Humans Inflammation / immunology Inflammation / metabolism Interleukin-1alpha / analysis Interleukin-1alpha / immunology* Interleukin-1alpha / metabolism Interleukin-1beta / immunology Lung / immunology* Lung / metabolism Lung Neoplasms / immunology* Lung Neoplasms / metabolism Mitogen-Activated Protein Kinase 1 / immunology Mitogen-Activated Protein Kinase 3 / immunology Nerve Tissue Proteins / immunology* Protein Transport TRPA1 Cation Channel Transient Receptor Potential Channels / immunology* Tumor Necrosis Factor-alpha / immunology |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |