論文 - 詳細
| RRC ID | 65930 |
|---|---|
| 著者 | Togo S, Katagiri N, Namba Y, Tulafu M, Nagahama K, Kadoya K, Takamochi K, Oh S, Suzuki K, Sakurai F, Mizuguchi H, Urata Y, Takahashi K. |
| タイトル | Sensitive detection of viable circulating tumor cells using a novel conditionally telomerase-selective replicating adenovirus in non-small cell lung cancer patients. |
| ジャーナル | Oncotarget |
| Abstract |
Circulating tumor cells (CTCs) have a crucial role in the clinical outcome of cancer patients. Detection of non-small cell lung cancer (NSCLC) using an antibody against epithelial cell adhesion molecule (EpCAM) in captured CTCs has low sensitivity; the loss of epithelial markers leads to underestimation of CTCs with mesenchymal phenotype. We propose a new approach for detection of viable CTCs, including those with epithelial-mesenchymal transition status (EMT-CTCs), using the new telomerase-specific replication-selective adenovirus (OBP-1101), TelomeScan F35. Peripheral venous blood samples and clinicopathological data were collected from 123 NSCLC patients. The sensitivity of CTC detection was 69.1%, and for patients with stage I, II, III and IV, it was 59.6%, 40.0%, 85.7%, and 75.0%, respectively. Among the EMT-CTC samples, 46% were vimentin positive and 39.0% of non-EMT-CTC samples were EpCAM positive. Patients testing positive for EMT-CTCs at baseline had poor response to chemotherapy (P = 0.025) and decreased progression-free survival (EMT-CTC positive vs. negative: 193 ± 47 days vs. 388 ± 47. days, P = 0.040) in comparison to those testing negative. TelomeScan F35 is a highly sensitive CTC detection system and will be a useful screening tool for early diagnosis of NSCLC patients. Mesenchymal-phenotype CTCs are crucial indicators of chemotherapeutic efficacy in NSCLC patients. |
| 巻・号 | 8(21) |
| ページ | 34884-34895 |
| 公開日 | 2017-5-23 |
| DOI | 10.18632/oncotarget.16818 |
| PII | 16818 |
| PMID | 28432274 |
| PMC | PMC5471019 |
| MeSH | A549 Cells Adenoviridae / physiology Carcinoma, Non-Small-Cell Lung / blood Carcinoma, Non-Small-Cell Lung / metabolism Carcinoma, Non-Small-Cell Lung / pathology* Cell Line, Tumor Disease-Free Survival Epithelial Cell Adhesion Molecule / metabolism Epithelial-Mesenchymal Transition Humans Lung Neoplasms / blood Lung Neoplasms / metabolism Lung Neoplasms / pathology* Male Neoplasm Staging Neoplastic Cells, Circulating / metabolism* Neoplastic Cells, Circulating / pathology Prognosis Survival Analysis Telomerase / metabolism* Vimentin / metabolism Virus Replication |
| IF | 5.168 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | PC-9(RCB4455) |