| RRC ID |
65930
|
| 著者 |
Togo S, Katagiri N, Namba Y, Tulafu M, Nagahama K, Kadoya K, Takamochi K, Oh S, Suzuki K, Sakurai F, Mizuguchi H, Urata Y, Takahashi K.
|
| タイトル |
Sensitive detection of viable circulating tumor cells using a novel conditionally telomerase-selective replicating adenovirus in non-small cell lung cancer patients.
|
| ジャーナル |
Oncotarget
|
| Abstract |
Circulating tumor cells (CTCs) have a crucial role in the clinical outcome of cancer patients. Detection of non-small cell lung cancer (NSCLC) using an antibody against epithelial cell adhesion molecule (EpCAM) in captured CTCs has low sensitivity; the loss of epithelial markers leads to underestimation of CTCs with mesenchymal phenotype. We propose a new approach for detection of viable CTCs, including those with epithelial-mesenchymal transition status (EMT-CTCs), using the new telomerase-specific replication-selective adenovirus (OBP-1101), TelomeScan F35. Peripheral venous blood samples and clinicopathological data were collected from 123 NSCLC patients. The sensitivity of CTC detection was 69.1%, and for patients with stage I, II, III and IV, it was 59.6%, 40.0%, 85.7%, and 75.0%, respectively. Among the EMT-CTC samples, 46% were vimentin positive and 39.0% of non-EMT-CTC samples were EpCAM positive. Patients testing positive for EMT-CTCs at baseline had poor response to chemotherapy (P = 0.025) and decreased progression-free survival (EMT-CTC positive vs. negative: 193 ± 47 days vs. 388 ± 47. days, P = 0.040) in comparison to those testing negative. TelomeScan F35 is a highly sensitive CTC detection system and will be a useful screening tool for early diagnosis of NSCLC patients. Mesenchymal-phenotype CTCs are crucial indicators of chemotherapeutic efficacy in NSCLC patients.
|
| 巻・号 |
8(21)
|
| ページ |
34884-34895
|
| 公開日 |
2017-5-23
|
| DOI |
10.18632/oncotarget.16818
|
| PII |
16818
|
| PMID |
28432274
|
| PMC |
PMC5471019
|
| MeSH |
A549 Cells
Adenoviridae / physiology
Carcinoma, Non-Small-Cell Lung / blood
Carcinoma, Non-Small-Cell Lung / metabolism
Carcinoma, Non-Small-Cell Lung / pathology*
Cell Line, Tumor
Disease-Free Survival
Epithelial Cell Adhesion Molecule / metabolism
Epithelial-Mesenchymal Transition
Humans
Lung Neoplasms / blood
Lung Neoplasms / metabolism
Lung Neoplasms / pathology*
Male
Neoplasm Staging
Neoplastic Cells, Circulating / metabolism*
Neoplastic Cells, Circulating / pathology
Prognosis
Survival Analysis
Telomerase / metabolism*
Vimentin / metabolism
Virus Replication
|
| IF |
5.168
|
| リソース情報 |
| ヒト・動物細胞 |
PC-9(RCB4455) |
