RRC ID 65930
Author Togo S, Katagiri N, Namba Y, Tulafu M, Nagahama K, Kadoya K, Takamochi K, Oh S, Suzuki K, Sakurai F, Mizuguchi H, Urata Y, Takahashi K.
Title Sensitive detection of viable circulating tumor cells using a novel conditionally telomerase-selective replicating adenovirus in non-small cell lung cancer patients.
Journal Oncotarget
Abstract Circulating tumor cells (CTCs) have a crucial role in the clinical outcome of cancer patients. Detection of non-small cell lung cancer (NSCLC) using an antibody against epithelial cell adhesion molecule (EpCAM) in captured CTCs has low sensitivity; the loss of epithelial markers leads to underestimation of CTCs with mesenchymal phenotype. We propose a new approach for detection of viable CTCs, including those with epithelial-mesenchymal transition status (EMT-CTCs), using the new telomerase-specific replication-selective adenovirus (OBP-1101), TelomeScan F35. Peripheral venous blood samples and clinicopathological data were collected from 123 NSCLC patients. The sensitivity of CTC detection was 69.1%, and for patients with stage I, II, III and IV, it was 59.6%, 40.0%, 85.7%, and 75.0%, respectively. Among the EMT-CTC samples, 46% were vimentin positive and 39.0% of non-EMT-CTC samples were EpCAM positive. Patients testing positive for EMT-CTCs at baseline had poor response to chemotherapy (P = 0.025) and decreased progression-free survival (EMT-CTC positive vs. negative: 193 ± 47 days vs. 388 ± 47. days, P = 0.040) in comparison to those testing negative. TelomeScan F35 is a highly sensitive CTC detection system and will be a useful screening tool for early diagnosis of NSCLC patients. Mesenchymal-phenotype CTCs are crucial indicators of chemotherapeutic efficacy in NSCLC patients.
Volume 8(21)
Pages 34884-34895
Published 2017-5-23
DOI 10.18632/oncotarget.16818
PII 16818
PMID 28432274
PMC PMC5471019
MeSH A549 Cells Adenoviridae / physiology Carcinoma, Non-Small-Cell Lung / blood Carcinoma, Non-Small-Cell Lung / metabolism Carcinoma, Non-Small-Cell Lung / pathology* Cell Line, Tumor Disease-Free Survival Epithelial Cell Adhesion Molecule / metabolism Epithelial-Mesenchymal Transition Humans Lung Neoplasms / blood Lung Neoplasms / metabolism Lung Neoplasms / pathology* Male Neoplasm Staging Neoplastic Cells, Circulating / metabolism* Neoplastic Cells, Circulating / pathology Prognosis Survival Analysis Telomerase / metabolism* Vimentin / metabolism Virus Replication
IF 5.168
Human and Animal Cells PC-9(RCB4455)