RRC ID 65938
著者 Takagishi T, Takehara M, Seike S, Miyamoto K, Kobayashi K, Nagahama M.
タイトル Clostridium perfringens α-toxin impairs erythropoiesis by inhibition of erythroid differentiation.
ジャーナル Sci Rep
Abstract Clostridium perfringens α-toxin induces hemolysis of erythrocytes from various species, but it has not been elucidated whether the toxin affects erythropoiesis. In this study, we treated bone marrow cells (BMCs) from mice with purified α-toxin and found that TER119+ erythroblasts were greatly decreased by the treatment. A variant α-toxin defective in enzymatic activities, phospholipase C and sphingomyelinase, had no effect on the population of erythroblasts, demonstrating that the decrease in erythroblasts was dependent of its enzymatic activities. α-Toxin reduced the CD71+TER119+ and CD71-TER119+ cell populations but not the CD71+TER119- cell population. In addition, α-toxin decreased the number of colony-forming unit erythroid colonies but not burst-forming unit erythroid colonies, indicating that α-toxin preferentially reduced mature erythroid cells compared with immature cells. α-Toxin slightly increased annexinV+ cells in TER119+ cells. Additionally, simultaneous treatment of BMCs with α-toxin and erythropoietin greatly attenuated the reduction of TER119+ erythroblasts by α-toxin. Furthermore, hemin-induced differentiation of human K562 erythroleukemia cells was impaired by α-toxin, whereas the treatment exhibited no apparent cytotoxicity. These results suggested that α-toxin mainly inhibited erythroid differentiation. Together, our results provide new insights into the biological activities of α-toxin, which might be important to understand the pathogenesis of C. perfringens infection.
巻・号 7(1)
ページ 5217
公開日 2017-7-12
DOI 10.1038/s41598-017-05567-8
PII 10.1038/s41598-017-05567-8
PMID 28701754
PMC PMC5507896
MeSH Animals Antigens, CD / metabolism Bacterial Toxins / toxicity* Blood Group Antigens / metabolism Calcium-Binding Proteins / toxicity* Cell Differentiation / drug effects* Cells, Cultured Erythroid Precursor Cells / drug effects Erythroid Precursor Cells / pathology* Erythropoiesis / drug effects* Humans K562 Cells Mice Mice, Inbred C57BL Receptors, Transferrin / metabolism Type C Phospholipases / toxicity*
IF 3.998
リソース情報
ヒト・動物細胞 K562(RCB0027)