RRC ID 65952
著者 Sehadova H, Takasu Y, Zaloudikova A, Lin YH, Sauman I, Sezutsu H, Rouhova L, Kodrik D, Zurovec M.
タイトル Functional Analysis of Adipokinetic Hormone Signaling in Bombyx mori.
ジャーナル Cells
Abstract Insect adipokinetic hormones (AKHs) are short peptides produced in the corpora cardiaca and are responsible for mobilizing energy stores from the fat body to the hemolymph. Three related peptides, AKH1, AKH2, and AKH/corazonin-related peptide (ACP) as well as three AKH receptors have been reported in Bombyx mori. AKH1 and AKH2 are specific for the AKHR1 receptor, whereas ACP interacts with the other two AKHRs. To assess the effect of the two silkworm AKHs and ACP in the regulation of energy homeostasis we examined the expression pattern of the three peptides and their receptors as well as their effect on the level of carbohydrates and lipids in the hemolymph. Our results support the hypothesis that only AKH1 and AKH2 peptides together with the AKHR1 receptor are involved in the maintenance of energy homeostasis. Because Bombyx AKHR1 (BmAKHR1) seems to be a true AKHR we generated its mutation. The BmAKHR1 mutant larvae display significantly lower carbohydrate and lipid levels in the hemolymph and reduced sensitivity to starvation. Our study clarifies the role of BmAKHR1 in energy homeostasis.
巻・号 9(12)
公開日 2020-12-11
DOI 10.3390/cells9122667
PII cells9122667
PMID 33322530
PMC PMC7764666
MeSH Animals Bombyx / growth & development Bombyx / metabolism* Carbohydrates / analysis Energy Metabolism Gene Expression Regulation Hemolymph / metabolism Insect Hormones / genetics Insect Hormones / metabolism* Insect Proteins / genetics Insect Proteins / metabolism Larva / metabolism Lipids / analysis Mutagenesis Neuropeptides / genetics Neuropeptides / metabolism Oligopeptides / genetics Oligopeptides / metabolism* Protein Isoforms / genetics Protein Isoforms / metabolism Pyrrolidonecarboxylic Acid / analogs & derivatives* Pyrrolidonecarboxylic Acid / metabolism Receptors, Glucagon / genetics Receptors, Glucagon / metabolism Signal Transduction*
IF 4.366
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