| RRC ID |
66022
|
| 著者 |
Rho SS, Kobayashi I, Oguri-Nakamura E, Ando K, Fujiwara M, Kamimura N, Hirata H, Iida A, Iwai Y, Mochizuki N, Fukuhara S.
|
| タイトル |
Rap1b Promotes Notch-Signal-Mediated Hematopoietic Stem Cell Development by Enhancing Integrin-Mediated Cell Adhesion.
|
| ジャーナル |
Dev Cell
|
| Abstract |
Hematopoietic stem cells (HSCs) emerge from hemogenic endothelium (HE) within the ventral portion of the dorsal aorta during vertebrate development. In zebrafish, Notch signaling induces HE specification from posterior lateral plate mesoderm (PLPM) cells as they migrate over the ventral surface of the somite. During migration, PLPM cells make close contact with Notch-ligand-expressing somitic cells to acquire HE identity. Herein, we show in zebrafish that the small GTPase Rap1b regulates HSC development by potentiating Notch-mediated HE specification. PLPM cells migrate toward the midline along the somite boundary where fibronectin accumulates. Rap1b stimulates integrin β1 to enhance PLPM cell adhesion to fibronectin localized at the somite boundary. Rap1b-induced integrin-β1-mediated adhesion to fibronectin leads to the spreading of PLPM cells to facilitate their physical contact with the Notch-ligand-expressing somitic cells, thereby promoting Notch-mediated HE specification. Thus, we have revealed an unexpected role of Rap1-induced integrin-mediated cell adhesion in HSC development.
|
| 巻・号 |
49(5)
|
| ページ |
681-696.e6
|
| 公開日 |
2019-6-3
|
| DOI |
10.1016/j.devcel.2019.03.023
|
| PII |
S1534-5807(19)30234-5
|
| PMID |
31006651
|
| MeSH |
Animals
Cell Adhesion*
Fibronectins / genetics
Fibronectins / metabolism*
Hematopoietic Stem Cells / cytology*
Hematopoietic Stem Cells / metabolism
Integrin beta1 / genetics
Integrin beta1 / metabolism*
Receptors, Notch / genetics
Receptors, Notch / metabolism*
Zebrafish
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism*
rap GTP-Binding Proteins / genetics
rap GTP-Binding Proteins / metabolism*
|
| IF |
10.092
|
| リソース情報 |
| ゼブラフィッシュ |
itgb1b mi371 |
