RRC ID 66058
著者 Beiriger A, Narayan S, Singh N, Prince V.
タイトル Development and migration of the zebrafish rhombencephalic octavolateral efferent neurons.
ジャーナル J Comp Neurol
Abstract In vertebrate animals, motor and sensory efferent neurons carry information from the central nervous system (CNS) to peripheral targets. These two types of efferent systems sometimes bear a close resemblance, sharing common segmental organization, axon pathways, and chemical messengers. Here, we focus on the development of the octavolateral efferent neurons (OENs) and their interactions with the closely-related facial branchiomotor neurons (FBMNs) in zebrafish. Using live-imaging approaches, we investigate the birth, migration, and projection patterns of OENs. We find that OENs are born in two distinct groups: a group of rostral efferent neurons (RENs) that arises in the fourth segment, or rhombomere (r4), of the hindbrain and a group of caudal efferent neurons (CENs) that arises in r5. Both RENs and CENs then migrate posteriorly through the hindbrain between 18 and 48 hrs postfertilization, alongside the r4-derived FBMNs. Like the FBMNs, migration of the r4-derived RENs depends on function of the segmental identity gene hoxb1a; unlike the FBMNs, however, both OEN populations move independently of prickle1b. Further, we investigate whether the previously described "pioneer" neuron that leads FBMN migration through the hindbrain is an r4-derived FBMN/REN or an r5-derived CEN. Our experiments verify that the pioneer is an r4-derived neuron and reaffirm its role in leading FBMN migration across the r4/5 border. In contrast, the r5-derived CENs migrate independently of the pioneer. Together, these results indicate that the mechanisms OENs use to navigate the hindbrain differ significantly from those employed by FBMNs.
巻・号 529(7)
ページ 1293-1307
公開日 2021-5-1
DOI 10.1002/cne.25021
PMID 32869305
PMC PMC8238524
MeSH Animals Cell Movement / physiology* Neurogenesis / physiology* Neurons, Efferent / physiology* Rhombencephalon / cytology* Rhombencephalon / physiology* Zebrafish
IF 2.801
リソース情報
ゼブラフィッシュ Tg(isl1:GFP) rw0