論文 - 詳細
| RRC ID | 66061 |
|---|---|
| 著者 | Xie ME, Adam Y, Fan LZ, Böhm UL, Kinsella I, Zhou D, Rozsa M, Singh A, Svoboda K, Paninski L, Cohen AE. |
| タイトル | High-fidelity estimates of spikes and subthreshold waveforms from 1-photon voltage imaging in vivo. |
| ジャーナル | Cell Rep |
| Abstract |
The ability to probe the membrane potential of multiple genetically defined neurons simultaneously would have a profound impact on neuroscience research. Genetically encoded voltage indicators are a promising tool for this purpose, and recent developments have achieved a high signal-to-noise ratio in vivo with 1-photon fluorescence imaging. However, these recordings exhibit several sources of noise and signal extraction remains a challenge. We present an improved signal extraction pipeline, spike-guided penalized matrix decomposition-nonnegative matrix factorization (SGPMD-NMF), which resolves supra- and subthreshold voltages in vivo. The method incorporates biophysical and optical constraints. We validate the pipeline with simultaneous patch-clamp and optical recordings from mouse layer 1 in vivo and with simulated and composite datasets with realistic noise. We demonstrate applications to mouse hippocampus expressing paQuasAr3-s or SomArchon1, mouse cortex expressing SomArchon1 or Voltron, and zebrafish spines expressing zArchon1. |
| 巻・号 | 35(1) |
| ページ | 108954 |
| 公開日 | 2021-4-6 |
| DOI | 10.1016/j.celrep.2021.108954 |
| PII | S2211-1247(21)00268-0 |
| PMID | 33826882 |
| PMC | PMC8095336 |
| MeSH | Action Potentials / physiology* Algorithms Animals Computer Simulation Hippocampus / physiology Imaging, Three-Dimensional* Mice, Transgenic Photons* Pyramidal Cells / physiology Reproducibility of Results Signal Transduction Zebrafish |
| IF | 8.109 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 44 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(vglut2a:Gal4) |