| RRC ID |
66062
|
| 著者 |
Lasseigne AM, Echeverry FA, Ijaz S, Michel JC, Martin EA, Marsh AJ, Trujillo E, Marsden KC, Pereda AE, Miller AC.
|
| タイトル |
Electrical synaptic transmission requires a postsynaptic scaffolding protein.
|
| ジャーナル |
Elife
|
| Abstract |
Electrical synaptic transmission relies on neuronal gap junctions containing channels constructed by Connexins. While at chemical synapses neurotransmitter-gated ion channels are critically supported by scaffolding proteins, it is unknown if channels at electrical synapses require similar scaffold support. Here, we investigated the functional relationship between neuronal Connexins and Zonula Occludens 1 (ZO1), an intracellular scaffolding protein localized to electrical synapses. Using model electrical synapses in zebrafish Mauthner cells, we demonstrated that ZO1 is required for robust synaptic Connexin localization, but Connexins are dispensable for ZO1 localization. Disrupting this hierarchical ZO1/Connexin relationship abolishes electrical transmission and disrupts Mauthner cell-initiated escape responses. We found that ZO1 is asymmetrically localized exclusively postsynaptically at neuronal contacts where it functions to assemble intercellular channels. Thus, forming functional neuronal gap junctions requires a postsynaptic scaffolding protein. The critical function of a scaffolding molecule reveals an unanticipated complexity of molecular and functional organization at electrical synapses.
|
| 巻・号 |
10
|
| 公開日 |
2021-4-28
|
| DOI |
10.7554/eLife.66898
|
| PII |
66898
|
| PMID |
33908867
|
| PMC |
PMC8081524
|
| MeSH |
Animals
Connexins / metabolism*
Electrical Synapses / physiology*
Synaptic Transmission / genetics*
Zebrafish / genetics
Zebrafish / physiology*
Zebrafish Proteins / genetics*
Zebrafish Proteins / metabolism
Zonula Occludens-1 Protein / genetics*
Zonula Occludens-1 Protein / metabolism
|
| IF |
7.08
|
| リソース情報 |
| ゼブラフィッシュ |
Tol-056 |
