RRC ID 66097
著者 Tanaka S, Nagashima H, Cruz M, Uchida T, Uotani T, Jiménez Abreu JA, Mahachai V, Vilaichone RK, Ratanachu-Ek T, Tshering L, Graham DY, Yamaoka Y.
タイトル Interleukin-17C in Human Helicobacter pylori Gastritis.
ジャーナル Infect Immun
Abstract The interleukin-17 (IL-17) family of cytokines (IL-17A to IL-17F) is involved in many inflammatory diseases. Although IL-17A is recognized as being involved in the pathophysiology of Helicobacter pylori-associated diseases, the role of other IL-17 cytokine family members remains unclear. Microarray analysis of IL-17 family cytokines was performed in H. pylori-infected and uninfected gastric biopsy specimens. IL-17C mRNA was upregulated approximately 4.5-fold in H. pylori-infected gastric biopsy specimens. This was confirmed by quantitative reverse transcriptase PCR in infected and uninfected gastric mucosa obtained from Bhutan and from the Dominican Republic. Immunohistochemical analysis showed that IL-17C expression in H. pylori-infected gastric biopsy specimens was predominantly localized to epithelial and chromogranin A-positive endocrine cells. IL-17C mRNA levels were also significantly greater among cagA-positive than cagA-negative H. pylori infections (P = 0.012). In vitro studies confirmed an increase in IL-17C mRNA and protein levels in cells infected with cagA-positive infections compared to cells infected with either cagA-negative or cag pathogenicity island (PAI) mutant. Chemical inhibition of IκB kinase (IKK), mitogen-activated protein extracellular signal-regulated kinase (MEK), and Jun N-terminal kinase (JNK) inhibited induction of IL-17C proteins in infected cells, whereas p38 inhibition had no effect on IL-17C protein secretion. In conclusion, H. pylori infection was associated with a significant increase in IL-17C expression in human gastric mucosa. The role of IL-17C in the pathogenesis of H. pylori-induced diseases remains to be determined.
巻・号 85(10)
公開日 2017-10-1
DOI 10.1128/IAI.00389-17
PII IAI.00389-17
PMID 28739826
PMC PMC5607407
MeSH Adolescent Adult Aged Aged, 80 and over Bacterial Proteins / genetics Bhutan Cell Line Dominican Republic Female Gastric Mucosa / immunology* Gastric Mucosa / metabolism Gastric Mucosa / microbiology Gastric Mucosa / pathology Gastritis / immunology* Gastritis / microbiology Gastritis / physiopathology Gene Regulatory Networks Genomic Islands Genotype Helicobacter Infections / epidemiology Helicobacter Infections / immunology* Helicobacter Infections / microbiology Helicobacter pylori / genetics Helicobacter pylori / immunology* Humans Interleukin-17 / genetics* Interleukin-17 / immunology* Male Middle Aged RNA, Messenger / genetics Real-Time Polymerase Chain Reaction Up-Regulation Young Adult
IF 3.201
リソース情報
ヒト・動物細胞 MKN45(RCB1001)