RRC ID 66144
著者 Ravanelli AM, Appel B.
タイトル Motor neurons and oligodendrocytes arise from distinct cell lineages by progenitor recruitment.
ジャーナル Genes Dev
Abstract During spinal cord development, ventral neural progenitor cells that express the transcription factors Olig1 and Olig2, called pMN progenitors, produce motor neurons and then oligodendrocytes. Whether motor neurons and oligodendrocytes arise from common or distinct progenitors in vivo is not known. Using zebrafish, we found that motor neurons and oligodendrocytes are produced sequentially by distinct progenitors that have distinct origins. When olig2(+) cells were tracked during the peak period of motor neuron formation, most differentiated as motor neurons without further cell division. Using time-lapse imaging, we found that, as motor neurons differentiated, more dorsally positioned neuroepithelial progenitors descended to the pMN domain and initiated olig2 expression. Inhibition of Hedgehog signaling during motor neuron differentiation blocked the ventral movement of progenitors, the progressive initiation of olig2 expression, and oligodendrocyte formation. We therefore propose that the motor neuron-to-oligodendrocyte switch results from Hedgehog-mediated recruitment of glial-fated progenitors to the pMN domain subsequent to neurogenesis.
巻・号 29(23)
ページ 2504-15
公開日 2015-12-1
DOI 10.1101/gad.271312.115
PII gad.271312.115
PMID 26584621
PMC PMC4691953
MeSH Animals Basic Helix-Loop-Helix Transcription Factors / genetics Basic Helix-Loop-Helix Transcription Factors / metabolism Cell Differentiation* Cell Lineage Cell Movement Embryo, Nonmammalian Gene Expression Regulation, Developmental Hedgehog Proteins / metabolism Motor Neurons / cytology* Nerve Tissue Proteins / genetics Nerve Tissue Proteins / metabolism Neuroepithelial Cells / cytology Neurogenesis / physiology Oligodendrocyte Transcription Factor 2 Oligodendroglia / cytology* Protein Structure, Tertiary Signal Transduction Stem Cells / cytology* Zebrafish / embryology* Zebrafish / genetics Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 9.527
リソース情報
ゼブラフィッシュ Tg(dbx1b:Cre) Tg(dbx1b:GFP)