| RRC ID |
66150
|
| 著者 |
Kohrogi K, Hino S, Sakamoto A, Anan K, Takase R, Araki H, Hino Y, Araki K, Sato T, Nakamura K, Nakao M.
|
| タイトル |
LSD1 defines erythroleukemia metabolism by controlling the lineage-specific transcription factors GATA1 and C/EBPα.
|
| ジャーナル |
Blood Adv
|
| Abstract |
Acute myeloid leukemia (AML) is a heterogenous malignancy characterized by distinct lineage subtypes and various genetic/epigenetic alterations. As with other neoplasms, AML cells have well-known aerobic glycolysis, but metabolic variations depending on cellular lineages also exist. Lysine-specific demethylase-1 (LSD1) has been reported to be crucial for human leukemogenesis, which is currently one of the emerging therapeutic targets. However, metabolic roles of LSD1 and lineage-dependent factors remain to be elucidated in AML cells. Here, we show that LSD1 directs a hematopoietic lineage-specific metabolic program in AML subtypes. Erythroid leukemia (EL) cells particularly showed activated glycolysis and high expression of LSD1 in both AML cell lines and clinical samples. Transcriptome, chromatin immunoprecipitation-sequencing, and metabolomic analyses revealed that LSD1 was essential not only for glycolysis but also for heme synthesis, the most characteristic metabolic pathway of erythroid origin. Notably, LSD1 stabilized the erythroid transcription factor GATA1, which directly enhanced the expression of glycolysis and heme synthesis genes. In contrast, LSD1 epigenetically downregulated the granulo-monocytic transcription factor C/EBPα. Thus, the use of LSD1 knockdown or chemical inhibitor dominated C/EBPα instead of GATA1 in EL cells, resulting in metabolic shifts and growth arrest. Furthermore, GATA1 suppressed the gene encoding C/EBPα that then acted as a repressor of GATA1 target genes. Collectively, we conclude that LSD1 shapes metabolic phenotypes in EL cells by balancing these lineage-specific transcription factors and that LSD1 inhibitors pharmacologically cause lineage-dependent metabolic remodeling.
|
| 巻・号 |
5(9)
|
| ページ |
2305-2318
|
| 公開日 |
2021-5-11
|
| DOI |
10.1182/bloodadvances.2020003521
|
| PII |
S2473-9529(21)00292-5
|
| PMID |
33929501
|
| PMC |
PMC8114557
|
| MeSH |
CCAAT-Enhancer-Binding Protein-alpha
GATA1 Transcription Factor / genetics
Histone Demethylases / genetics
Humans
Leukemia, Erythroblastic, Acute* / genetics
Proto-Oncogene Proteins
Transcription Factors
|
| IF |
4.91
|
| リソース情報 |
| 遺伝子材料 |
pENTR4-H1tetOx1 (RDB07916) |
| ヒト・動物細胞 |
HL-60(RCB3683) |
