Reference - Detail
| RRC ID | 66164 |
|---|---|
| Author | Ogawa S, Kubo H, Murayama Y, Kubota T, Yubakami M, Matsumoto T, Ohashi T, Okamoto K, Kuriki Y, Hanaoka K, Urano Y, Otsuji E. |
| Title | Matrix metalloprotease-14 is a target enzyme for detecting peritoneal metastasis in gastric cancer. |
| Journal | Photodiagnosis Photodyn Ther |
| Abstract |
BACKGROUND:Accurate diagnosis of peritoneal metastasis in gastric cancer (GC) is important to determine the appropriate treatment. This study aimed to examine whether matrix metalloprotease-14 (MMP-14) was a candidate enzyme in fluorescence imaging for the diagnosis of peritoneal metastasis in GC. METHODS:GC and normal peritoneal (NP) tissues from 96 and 20 patients, respectively were evaluated for MMP-14 expression. Live cell imaging of GC cell lines (NUGC4, MKN45, MKN74, HGC-27, and Kato-III) was performed using the MMP-14-activatable fluorescence probe; BODIPY-MMP. Furthermore, the overall survival (OS) was calculated in all patients (n = 96). RESULTS:MMP-14 expression was significantly higher in GC tissues (median: 3.57 ng/mg protein; range:0.64-24.4 ng/mg protein) than in NP tissues (median: 1.34 ng/mg protein; median: 0.53-3.09 ng/mg protein) (P < 0.01). Receiver operating characteristic curves showed that the area under the curve, sensitivity, and specificity were 0.907, 84.4%, and 90.0%, respectively. In live cell imaging using the BODIPY-MMP, fluorescence was observed in five GC cell lines. In the analysis of OS, the high expression of the MMP-14 group had a significantly poorer OS rate than the low expression of the MMP-14 group (P = 0.02). In the multivariate analyses, MMP-14 expression was an independent risk factor for OS (hazard ratio: 2.33; 95 % confidence interval: 1.05-5.45; P = 0.04). CONCLUSION:MMP-14 is a promising enzyme in intraoperative fluorescence imaging for peritoneal metastasis in GC, especially in patients with poor prognosis. |
| Volume | 35 |
| Pages | 102420 |
| Published | 2021-9-1 |
| DOI | 10.1016/j.pdpdt.2021.102420 |
| PII | S1572-1000(21)00247-7 |
| PMID | 34242818 |
| MeSH | Biomarkers, Tumor Humans Matrix Metalloproteinase 14 Peritoneal Neoplasms* / diagnostic imaging Photochemotherapy* / methods Photosensitizing Agents Prognosis Stomach Neoplasms* / diagnostic imaging |
| IF | 2.894 |
| Resource | |
| Human and Animal Cells | NUGC-4(RCB1939) MKN45(RCB1001) MKN74(RCB1002) HGC-27(RCB0500) |