Reference - Detail
| RRC ID | 66170 |
|---|---|
| Author | Cho G, Lee E, Kim J. |
| Title | Structural insights into phosphatidylethanolamine formation in bacterial membrane biogenesis. |
| Journal | Sci Rep |
| Abstract |
Phosphatidylethanolamine (PE), a major component of the cellular membrane across all domains of life, is synthesized exclusively by membrane-anchored phosphatidylserine decarboxylase (PSD) in most bacteria. The enzyme undergoes auto-cleavage for activation and utilizes the pyruvoyl moiety to form a Schiff base intermediate with PS to facilitate decarboxylation. However, the structural basis for self-maturation, PS binding, and decarboxylation processes directed by PSD remain unclear. Here, we present X-ray crystal structures of PSD from Escherichia coli, representing an apo form and a PE-bound complex, in which the phospholipid is chemically conjugated to the essential pyruvoyl residue, mimicking the Schiff base intermediate. The high-resolution structures of PE-complexed PSD clearly illustrate extensive hydrophobic interactions with the fatty acyl chains of the phospholipid, providing insights into the broad specificity of the enzyme over a wide range of cellular PS. Furthermore, these structures strongly advocate the unique topology of the enzyme in a lipid bilayer environment, where the enzyme associates with cell membranes in a monotopic fashion via the N-terminal domain composed of three amphipathic helices. Lastly, mutagenesis analyses reveal that E. coli PSD primarily employs D90/D142-H144-S254 to achieve auto-cleavage for the proenzyme maturation, where D90 and D142 act in complementary to each other. |
| Volume | 11(1) |
| Pages | 5785 |
| Published | 2021-3-11 |
| DOI | 10.1038/s41598-021-85195-5 |
| PII | 10.1038/s41598-021-85195-5 |
| PMID | 33707636 |
| PMC | PMC7952604 |
| MeSH | Apoproteins / chemistry Carboxy-Lyases / chemistry Carboxy-Lyases / metabolism Cell Membrane / metabolism* Crystallography, X-Ray Escherichia coli / enzymology Escherichia coli / metabolism* Models, Molecular Phosphatidylethanolamines / chemistry* Phosphoserine / metabolism Protein Binding Protein Domains |
| IF | 3.998 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Prokaryotes E. coli | JW4121-AM |