RRC ID 6618
著者 Syudo M, Yamada S, Yanagiguchi K, Matsunaga T, Hayashi Y.
タイトル Early gene expression analyzed by a genome microarray and real-time PCR in osteoblasts cultured with a 4-META/MMA-TBB adhesive resin sealer.
ジャーナル Oral Surg Oral Med Oral Pathol Oral Radiol Endod
Abstract OBJECTIVES:Adhesive resin sealer systems have been applied in endodontics to seal the root canal system. This study was designed to confirm the mechanism of intracellular molecular events in an in vitro cell culture system with a 4-methacryloxyethyl trimellitate anhydride/methylmethacrylate-tri-n-butyl borane (4-META/MMA-TBB) adhesive resin sealer.
STUDY DESIGN:The gene expression patterns relating to cell growth and differentiation were examined using a human genome expression microarray and real-time polymerase chain reaction analyses in hard tissue-forming osteoblasts cultured with and without a 4-META/MMA-TBB resin sealer.
RESULTS:There was no significant difference in the cell number between the control and adhesive sealer groups. An increased expression of integrin beta, transforming growth factor beta-related protein, craniofacial development protein 1, and PI3K genes was demonstrated. The integrin beta and PI3K genes showed extremely high ratios.
CONCLUSIONS:The signal transduction pathway, at least through the PI3K/Akt cascade for cell proliferation and differentiation, can be controlled by some components of this type of adhesive resin sealer.
巻・号 107(3)
ページ e77-81
公開日 2009-3-1
DOI 10.1016/j.tripleo.2008.10.020
PII S1079-2104(08)00807-X
PMID 19157924
MeSH Acrylic Resins / pharmacology* Cell Line, Tumor Class II Phosphatidylinositol 3-Kinases Humans Integrin beta3 / biosynthesis MAP Kinase Signaling System / drug effects* Materials Testing Nuclear Proteins Oligonucleotide Array Sequence Analysis Osteoblasts / drug effects* Osteoblasts / metabolism* Phosphatidylinositol 3-Kinases / biosynthesis Phosphoproteins / biosynthesis Polymerase Chain Reaction Repressor Proteins / biosynthesis Root Canal Filling Materials / pharmacology*
引用数 3
WOS 分野 DENTISTRY, ORAL SURGERY & MEDICINE
リソース情報
ヒト・動物細胞