論文 - 詳細
| RRC ID | 66195 |
|---|---|
| 著者 | Toyotake Y, Nishiyama M, Yokoyama F, Ogawa T, Kawamoto J, Kurihara T. |
| タイトル | A Novel Lysophosphatidic Acid Acyltransferase of Escherichia coli Produces Membrane Phospholipids with a cis-vaccenoyl Group and Is Related to Flagellar Formation. |
| ジャーナル | Biomolecules |
| Abstract |
Lysophosphatidic acid acyltransferase (LPAAT) introduces fatty acyl groups into the sn-2 position of membrane phospholipids (PLs). Various bacteria produce multiple LPAATs, whereas it is believed that Escherichia coli produces only one essential LPAAT homolog, PlsC-the deletion of which is lethal. However, we found that E. coli possesses another LPAAT homolog named YihG. Here, we show that overexpression of YihG in E. coli carrying a temperature-sensitive mutation in plsC allowed its growth at non-permissive temperatures. Analysis of the fatty acyl composition of PLs from the yihG-deletion mutant (∆yihG) revealed that endogenous YihG introduces the cis-vaccenoyl group into the sn-2 position of PLs. Loss of YihG did not affect cell growth or morphology, but ∆yihG cells swam well in liquid medium in contrast to wild-type cells. Immunoblot analysis showed that FliC was highly expressed in ∆yihG cells, and this phenotype was suppressed by expression of recombinant YihG in ∆yihG cells. Transmission electron microscopy confirmed that the flagellar structure was observed only in ∆yihG cells. These results suggest that YihG has specific functions related to flagellar formation through modulation of the fatty acyl composition of membrane PLs. |
| 巻・号 | 10(5) |
| 公開日 | 2020-5-11 |
| DOI | 10.3390/biom10050745 |
| PII | biom10050745 |
| PMID | 32403425 |
| PMC | PMC7277886 |
| MeSH | Acyltransferases / metabolism* Escherichia coli / enzymology* Escherichia coli / growth & development Escherichia coli / ultrastructure Escherichia coli Proteins / metabolism* Flagella / metabolism* Flagella / ultrastructure Membrane Lipids / metabolism* Mutation / genetics Phospholipids / metabolism* Substrate Specificity Temperature |
| IF | 4.082 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 原核生物(大腸菌) | ME9062 |