| RRC ID |
66195
|
| 著者 |
Toyotake Y, Nishiyama M, Yokoyama F, Ogawa T, Kawamoto J, Kurihara T.
|
| タイトル |
A Novel Lysophosphatidic Acid Acyltransferase of Escherichia coli Produces Membrane Phospholipids with a cis-vaccenoyl Group and Is Related to Flagellar Formation.
|
| ジャーナル |
Biomolecules
|
| Abstract |
Lysophosphatidic acid acyltransferase (LPAAT) introduces fatty acyl groups into the sn-2 position of membrane phospholipids (PLs). Various bacteria produce multiple LPAATs, whereas it is believed that Escherichia coli produces only one essential LPAAT homolog, PlsC-the deletion of which is lethal. However, we found that E. coli possesses another LPAAT homolog named YihG. Here, we show that overexpression of YihG in E. coli carrying a temperature-sensitive mutation in plsC allowed its growth at non-permissive temperatures. Analysis of the fatty acyl composition of PLs from the yihG-deletion mutant (∆yihG) revealed that endogenous YihG introduces the cis-vaccenoyl group into the sn-2 position of PLs. Loss of YihG did not affect cell growth or morphology, but ∆yihG cells swam well in liquid medium in contrast to wild-type cells. Immunoblot analysis showed that FliC was highly expressed in ∆yihG cells, and this phenotype was suppressed by expression of recombinant YihG in ∆yihG cells. Transmission electron microscopy confirmed that the flagellar structure was observed only in ∆yihG cells. These results suggest that YihG has specific functions related to flagellar formation through modulation of the fatty acyl composition of membrane PLs.
|
| 巻・号 |
10(5)
|
| 公開日 |
2020-5-11
|
| DOI |
10.3390/biom10050745
|
| PII |
biom10050745
|
| PMID |
32403425
|
| PMC |
PMC7277886
|
| MeSH |
Acyltransferases / metabolism*
Escherichia coli / enzymology*
Escherichia coli / growth & development
Escherichia coli / ultrastructure
Escherichia coli Proteins / metabolism*
Flagella / metabolism*
Flagella / ultrastructure
Membrane Lipids / metabolism*
Mutation / genetics
Phospholipids / metabolism*
Substrate Specificity
Temperature
|
| IF |
4.082
|
| リソース情報 |
| 原核生物(大腸菌) |
ME9062 |
