論文 - 詳細
| RRC ID | 66209 |
|---|---|
| 著者 | Huergo LF, Araújo GAT, Santos ASR, Gerhardt ECM, Pedrosa FO, Souza EM, Forchhammer K. |
| タイトル | The NADP-dependent malic enzyme MaeB is a central metabolic hub controlled by the acetyl-CoA to CoASH ratio. |
| ジャーナル | Biochim Biophys Acta Proteins Proteom |
| Abstract |
Malic enzymes participate in key metabolic processes, the MaeB-like malic enzymes carry a catalytic inactive phosphotransacetylase domain whose function remains elusive. Here we show that acetyl-CoA directly binds and inhibits MaeB-like enzymes with a saturable profile under physiological relevant acetyl-CoA concentrations. A MaeB-like enzyme from the nitrogen-fixing bacterium Azospirillum brasilense, namely AbMaeB1, binds both acetyl-CoA and unesterified CoASH in a way that inhibition of AbMaeB1 by acetyl-CoA is relieved by increasing CoASH concentrations. Hence, AbMaeB1 senses the acetyl-CoA/CoASH ratio. We revisited E. coli MaeB regulation to determine the inhibitory constant for acetyl-CoA. Our data support that the phosphotransacetylase domain of MaeB-like enzymes senses acetyl-CoA to dictate the fate of carbon distribution at the phosphoenol-pyruvate / pyruvate / oxaloacetate metabolic node. |
| 巻・号 | 1868(9) |
| ページ | 140462 |
| 公開日 | 2020-9-1 |
| DOI | 10.1016/j.bbapap.2020.140462 |
| PII | S1570-9639(20)30109-6 |
| PMID | 32485238 |
| MeSH | Acetyl Coenzyme A / metabolism* Azospirillum brasilense / genetics Azospirillum brasilense / metabolism Bacteria / metabolism Coenzyme A / metabolism* Escherichia coli / genetics Escherichia coli / metabolism Malate Dehydrogenase / genetics Malate Dehydrogenase / metabolism* Malates / metabolism* NADP / metabolism* Phosphate Acetyltransferase / metabolism |
| IF | 2.609 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 原核生物(大腸菌) | ASKA collection |