RRC ID |
66214
|
著者 |
Hans S, Haby B, Krausch N, Barz T, Neubauer P, Cruz-Bournazou MN.
|
タイトル |
Automated Conditional Screening of Multiple Escherichia coli Strains in Parallel Adaptive Fed-Batch Cultivations.
|
ジャーナル |
Bioengineering (Basel)
|
Abstract |
In bioprocess development, the host and the genetic construct for a new biomanufacturing process are selected in the early developmental stages. This decision, made at the screening scale with very limited information about the performance in larger reactors, has a major influence on the efficiency of the final process. To overcome this, scale-down approaches during screenings that show the real cell factory performance at industrial-like conditions are essential. We present a fully automated robotic facility with 24 parallel mini-bioreactors that is operated by a model-based adaptive input design framework for the characterization of clone libraries under scale-down conditions. The cultivation operation strategies are computed and continuously refined based on a macro-kinetic growth model that is continuously re-fitted to the available experimental data. The added value of the approach is demonstrated with 24 parallel fed-batch cultivations in a mini-bioreactor system with eight different Escherichia coli strains in triplicate. The 24 fed-batch cultivations were run under the desired conditions, generating sufficient information to define the fastest-growing strain in an environment with oscillating glucose concentrations similar to industrial-scale bioreactors.
|
巻・号 |
7(4)
|
公開日 |
2020-11-11
|
DOI |
10.3390/bioengineering7040145
|
PII |
bioengineering7040145
|
PMID |
33187191
|
PMC |
PMC7711848
|
リソース情報 |
原核生物(大腸菌) |
JW0554-KC
JW3975-KC
JW1907-KC
JW2076-KC
JW2082-KC
JW2943-KC |