RRC ID 66214
著者 Hans S, Haby B, Krausch N, Barz T, Neubauer P, Cruz-Bournazou MN.
タイトル Automated Conditional Screening of Multiple Escherichia coli Strains in Parallel Adaptive Fed-Batch Cultivations.
ジャーナル Bioengineering (Basel)
Abstract In bioprocess development, the host and the genetic construct for a new biomanufacturing process are selected in the early developmental stages. This decision, made at the screening scale with very limited information about the performance in larger reactors, has a major influence on the efficiency of the final process. To overcome this, scale-down approaches during screenings that show the real cell factory performance at industrial-like conditions are essential. We present a fully automated robotic facility with 24 parallel mini-bioreactors that is operated by a model-based adaptive input design framework for the characterization of clone libraries under scale-down conditions. The cultivation operation strategies are computed and continuously refined based on a macro-kinetic growth model that is continuously re-fitted to the available experimental data. The added value of the approach is demonstrated with 24 parallel fed-batch cultivations in a mini-bioreactor system with eight different Escherichia coli strains in triplicate. The 24 fed-batch cultivations were run under the desired conditions, generating sufficient information to define the fastest-growing strain in an environment with oscillating glucose concentrations similar to industrial-scale bioreactors.
巻・号 7(4)
公開日 2020-11-11
DOI 10.3390/bioengineering7040145
PII bioengineering7040145
PMID 33187191
PMC PMC7711848
リソース情報
原核生物(大腸菌) JW0554-KC JW3975-KC JW1907-KC JW2076-KC JW2082-KC JW2943-KC