| RRC ID |
6627
|
| 著者 |
Kawase R, Ishiwata T, Matsuda Y, Onda M, Kudo M, Takeshita T, Naito Z.
|
| タイトル |
Expression of fibroblast growth factor receptor 2 IIIc in human uterine cervical intraepithelial neoplasia and cervical cancer.
|
| ジャーナル |
Int J Oncol
|
| Abstract |
Fibroblast growth factor receptors (FGFRs) 1-3 have IIIb and IIIc isoforms, and we reported that FGFR2 IIIb is highly expressed in cervical keratinizing squamous cell carcinoma (SCC). In this study, we determined the expression and roles of FGFR2 IIIc in cervical intraepithelial neoplasia (CIN) and cervical cancer. In CINs 1 and 2, FGFR2 IIIc was found to be localized at the basal to lower two-thirds of the squamous epithelium, whereas it was localized in most of the squamous epithelium, except for the superficial layer in CIN 3. In situ hybridization (ISH) analysis showed that the expression patterns of FGFR2 IIIc mRNA are similar to those of FGFR2 IIIc protein in CINs. The FGFR2 IIIc protein was detected in all invasive cervical cancer patients (29 cases) and its mRNA was found to be strongly expressed in the invasive front of cancer cell nests. FGFR2 IIIc cDNA was stably transfected into CaSki cells, which are derived from a cervical SCC. The growth rates of the CaSki cells were higher than those of Mock cells in vitro, and the CaSki cells tended to form larger subcutaneous tumors in nude mice. These findings suggest that FGFR2 IIIc plays important roles in carcinogenesis and growth of cervical cancer cells. Anti-FGFR2 IIIc therapies may represent therapeutic strategies for inhibiting the growth of CIN and cervical cancer.
|
| 巻・号 |
36(2)
|
| ページ |
331-40
|
| 公開日 |
2010-2-1
|
| PMID |
20043066
|
| MeSH |
Adult
Aged
Animals
Biomarkers, Tumor / analysis*
Blotting, Western
Cervical Intraepithelial Neoplasia / genetics
Cervical Intraepithelial Neoplasia / metabolism*
Cervical Intraepithelial Neoplasia / pathology
Female
Gene Expression
Gene Expression Profiling
Humans
Immunohistochemistry
Mice
Middle Aged
Neoplasm Staging
RNA, Messenger / analysis
Receptor, Fibroblast Growth Factor, Type 2 / biosynthesis*
Receptor, Fibroblast Growth Factor, Type 2 / genetics
Reverse Transcriptase Polymerase Chain Reaction
Uterine Cervical Neoplasms / genetics
Uterine Cervical Neoplasms / metabolism*
Uterine Cervical Neoplasms / pathology
|
| IF |
3.899
|
| 引用数 |
23
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
Ca Ski(RCB1947) |
