Reference - Detail
| RRC ID | 66338 |
|---|---|
| Author | Onishi K, Mochizuki J, Sato A, Goto A, Sashihara T. |
| Title | Total RNA and genomic DNA of Lactobacillus gasseri OLL2809 induce interleukin-12 production in the mouse macrophage cell line J774.1 via toll-like receptors 7 and 9. |
| Journal | BMC Microbiol |
| Abstract |
BACKGROUND:Lactobacillus gasseri OLL2809 can highly induce interleukin (IL)-12 production in immune cells. Even though beneficial properties of this strain for both humans and animals have been reported, the mechanism by which the bacteria induces the production of IL-12 in immune cells remains elusive. In this study, we investigated the mechanism of induction of IL-12 using a mouse macrophage cell line J774.1. RESULTS:Inhibition of phagocytosis of L. gasseri OLL2809, and myeloid differentiation factor 88 and Toll-like receptors (TLRs) 7 and 9 signalling attenuated IL-12 production in J774.1 cells. Total RNA and genomic DNA of L. gasseri OLL2809, when transferred to the J774.1 cells, also induced IL-12 production. The difference in the IL-12-inducing activity of Lactobacilli is attributed to the susceptibility to phagocytosis, but not to a difference in the total RNA and genomic DNA of each strain. CONCLUSION:We concluded that total RNA and genomic DNA of phagocytosed L. gasseri OLL2809 induce IL-12 production in J774.1 cell via TLRs 7 and 9, and the high IL-12-inducing activity of L. gasseri OLL2809 is due to its greater susceptibility to phagocytosis. |
| Volume | 20(1) |
| Pages | 217 |
| Published | 2020-7-20 |
| DOI | 10.1186/s12866-020-01900-w |
| PII | 10.1186/s12866-020-01900-w |
| PMID | 32689952 |
| PMC | PMC7372888 |
| MeSH | Animals Cell Culture Techniques Cell Line DNA, Bacterial / immunology* Genome, Bacterial Interleukin-12 / metabolism* Lactobacillus gasseri / genetics* Lactobacillus gasseri / immunology Macrophages / cytology Macrophages / immunology* Macrophages / microbiology Membrane Glycoproteins / metabolism* Mice Myeloid Differentiation Factor 88 / metabolism Phagocytosis RNA, Bacterial / immunology* Signal Transduction Toll-Like Receptor 7 / metabolism* Toll-Like Receptor 9 / metabolism* Up-Regulation |
| Resource | |
| General Microbes | JCM1131 JCM1149 JCM1185 |