RRC ID 6634
著者 Hata T, Iwasaki J, Hishikawa S, Fujimoto Y, Uemoto S, Kobayashi E.
タイトル Development of a portocaval shunt using a small intestinal segment in rats.
ジャーナル Microsurgery
Abstract The transjugular portosystemic shunt, widely used to treat portal hypertension today, may increase the risk of encephalopathy and reduce effective hepatic flow. To address these issues, a strategy to produce a portocaval shunt (PCS) with hepatic function using intestinal grafts was conceived, and rat models were developed. We transplanted ileal grafts from wild-type and luciferase transgenic Lewis rats to wild-type Lewis rats, anastomosing the graft mesenteric artery (SMA) and portal vein (PV) to the recipient PV trunk and inferior vena cava, respectively. Recipient survival was significantly longer in the partial PCS model, in which the graft SMA was anastomosed to the recipient PV trunk in an end-to-side fashion, than in the total PCS model, with the end-to-end anastomosis. In the partial PCS model, histological and luminescence analyses showed graft survival for 1 month. These results suggest that intestinal grafts can be maintained in the particular conditions required for our strategy.
巻・号 30(4)
ページ 302-6
公開日 2010-5-1
DOI 10.1002/micr.20751
PMID 20309853
MeSH Anastomosis, Surgical / methods Animals Biopsy, Needle Female Graft Rejection Graft Survival Hypertension, Portal / surgery Ileum / blood supply Ileum / pathology* Ileum / transplantation* Immunohistochemistry Luminescent Measurements Male Models, Animal Portacaval Shunt, Surgical / adverse effects Portacaval Shunt, Surgical / methods* Portasystemic Shunt, Surgical / methods Probability Random Allocation Rats Rats, Inbred Lew Rats, Transgenic Regional Blood Flow / physiology Risk Factors Statistics, Nonparametric Tissue Transplantation / methods*
IF 1.996
引用数 1
WOS 分野 SURGERY
リソース情報
ラット LEW-Tg(Gt(ROSA)26Sor-luc)11Jmsk(strainID=649) LEW-Tg(Gt(ROSA)26Sor-luc)21Jmsk(strainID=650)