| RRC ID |
6635
|
| 著者 |
Iwasaki J, Hata T, Hishikawa S, Fujimoto Y, Uemoto S, Murakami T, Kobayashi E.
|
| タイトル |
Use of rat segmental intestine for fetal pancreatic transplantation.
|
| ジャーナル |
Microsurgery
|
| Abstract |
It is thought that the small intestine may provide a scaffold for pancreas regeneration. Herein, we investigated whether fetal pancreatic tissue could be transplanted into the segmental intestine in rats. Fetal pancreases from firefly luciferase transgenic Lewis rat embryos (embryonic day 14.5 and 15.5) were transplanted into streptozotocin (STZ)-induced diabetic wild-type Lewis rats. As a scaffold for pancreatic development, rat small intestinal segments were utilized after the removal of mucosa, and fetal pancreases were grafted into the luminal surface through the stoma. We also transplanted fetal pancreases into the omentum. The survival of transplanted fetal pancreases was monitored by luciferase-derived photons and blood glucose levels. Transplanted fetal pancreas-derived photons were stable for 28 days, suggesting that transplanted fetal pancreatic tissues survived and that their intestinal blood supply was maintained.
|
| 巻・号 |
30(4)
|
| ページ |
296-301
|
| 公開日 |
2010-5-1
|
| DOI |
10.1002/micr.20771
|
| PMID |
20309851
|
| MeSH |
Animals
Blood Glucose / analysis
Blood Glucose / metabolism*
Diabetes Mellitus, Experimental / surgery*
Disease Models, Animal
Female
Fetal Tissue Transplantation / adverse effects
Fetal Tissue Transplantation / methods*
Graft Rejection
Graft Survival
Jejunum / pathology
Jejunum / surgery*
Male
Pancreas Transplantation / adverse effects
Pancreas Transplantation / methods*
Random Allocation
Rats
Rats, Inbred Lew
Rats, Transgenic
Risk Factors
Statistics, Nonparametric
Survival Rate
|
| IF |
1.996
|
| 引用数 |
1
|
|
WOS 分野
|
SURGERY
|
| リソース情報 |
| ラット |
LEW-Tg(Gt(ROSA)26Sor-luc)11Jmsk(strainID=649)
LEW-Tg(Gt(ROSA)26Sor-luc)22Jmsk |
