論文 - 詳細
| RRC ID | 66379 |
|---|---|
| 著者 | Mamiya A, Sakka M, Kosugi A, Katsuzaki H, Tanaka A, Kunitake E, Kimura T, Sakka K. |
| タイトル | Significance of a family-6 carbohydrate-binding module in a modular feruloyl esterase for removing ferulic acid from insoluble wheat arabinoxylan. |
| ジャーナル | Enzyme Microb Technol |
| Abstract |
Ruminiclostridium josui Fae1A is a modular enzyme consisting of an N-terminal signal peptide, family-1 carbohydrate esterase module (CE1), family-6 carbohydrate-binding module (CBM6), and dockerin module in that order. Recombinant CE1 and CBM6 polypeptides were collectively and separately produced as RjFae1A, RjCE1, and RjCBM6. RjFae1A showed higher feruloyl esterase activity than RjCE1 towards insoluble wheat arabinoxylan, but the latter was more active towards small synthetic substrates than the former. This suggests that CBM6 in RjFae1A plays an important role in releasing ferulic acid from the native substrate. RjCBM6 showed a higher affinity for soluble wheat arabinoxylan than for rye arabinoxylan and beechwood xylan in native affinity polyacrylamide gel electrophoresis. Isothermal titration calorimetry analysis demonstrated that RjCBM6 recognized a xylopyranosyl residue at the nonreducing ends of xylooligosaccharides. Moreover, it showed exceptional affinity for 23-α-l-arabinofuranosyl-xylotriose (A2XX) among the tested branched arabinoxylooligosaccharides. Fluorometric titration analysis demonstrated that xylobiose and A2XX competitively bound to RjCBM6, and both bound to the same site in RjCBM6. RjCBM6's preference for the xylopyranosyl residue at the nonreducing end of xylan chains explains why the positive effect of CBM6 on RjFae1A activity was observed only during short incubation but not after extended incubation. |
| 巻・号 | 138 |
| ページ | 109546 |
| 公開日 | 2020-8-1 |
| DOI | 10.1016/j.enzmictec.2020.109546 |
| PII | S0141-0229(20)30039-9 |
| PMID | 32527521 |
| MeSH | Bacterial Proteins / chemistry Bacterial Proteins / genetics Bacterial Proteins / metabolism Carboxylic Ester Hydrolases / chemistry* Carboxylic Ester Hydrolases / genetics Carboxylic Ester Hydrolases / metabolism* Clostridiales / enzymology Coumaric Acids / metabolism* Glucuronates / chemistry Glucuronates / metabolism Oligosaccharides / chemistry Oligosaccharides / metabolism Polysaccharides / metabolism Protein Binding Protein Domains Recombinant Proteins / chemistry Recombinant Proteins / genetics Recombinant Proteins / metabolism Secale / chemistry Substrate Specificity Triticum / chemistry* Xylans / metabolism* Xylose / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM17888 |