Reference - Detail
| RRC ID | 66445 |
|---|---|
| Author | Sakamoto M, Ikeyama N, Toyoda A, Murakami T, Mori H, Iino T, Ohkuma M. |
| Title | Dialister hominis sp. nov., isolated from human faeces. |
| Journal | Int J Syst Evol Microbiol |
| Abstract |
An obligately anaerobic, Gram-stain-negative, rod or coccobacilli organism was isolated from a faecal sample of a healthy Japanese woman. In the 16S rRNA gene sequence analysis, strain 5BBH33T showed the highest 16S rRNA gene sequence similarity to Dialister succinatiphilus YIT 11850T (95.9 %), Dialister propionicifaciens ADV 1053.03T (94.3 %), Dialister micraerophilus DSM 19965T (93.1 %), Dialister invisus DSM 15470T (92.5 %) and Dialister pneumosintes ATCC 33048T (91.4 %). The hsp60 gene sequence analysis also revealed strain 5BBH33T had relatively low hsp60 gene sequence similarities (74.4-85.3 %) to other Dialister species. Strain 5BBH33T showed 21.8-23.9 % in silico DNA-DNA hybridization values with other Dialister species. In addition, the average nucleotide identity values between strain 5BBH33T and other Dialister species ranged from 68.7-74.2 %, indicating that this strain should be considered as new species based on whole-genome relatedness. Strain 5BBH33T was asaccharolytic and largely unreactive for commercial kit. However, its growth was enhanced by adding 1 % (w/v) succinate to the medium; strain 5BBH33T was able to decarboxylate succinate to propionate. The strain 5BBH33T genome contained the enzymes involved in succinate utilization. These results improve our understanding of succinate-utilizing bacteria. On the basis of the collected data, strain 5BBH33T represents a novel species in the genus Dialister, for which the name Dialister hominis sp. nov. is proposed. The type strain of D. hominis is 5BBH33T (=JCM 33369T=DSM 109768T). |
| Volume | 70(1) |
| Pages | 589-595 |
| Published | 2020-1-1 |
| DOI | 10.1099/ijsem.0.003797 |
| PMID | 31661048 |
| MeSH | Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Feces / microbiology* Female Humans Japan Nucleic Acid Hybridization Phylogeny* RNA, Ribosomal, 16S / genetics Sequence Analysis, DNA Veillonellaceae / classification* Veillonellaceae / isolation & purification |
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| The most frequently cited source | Blogs |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| General Microbes | JCM33369 JCM10004 JCM15077 JCM17566 JCM17567 JCM17568 |