| RRC ID |
66479
|
| 著者 |
Shimizu Y, Konno Y, Tomita Y.
|
| タイトル |
Wickerhamomyces psychrolipolyticus f.a., sp. nov., a novel yeast species producing two kinds of lipases with activity at different temperatures.
|
| ジャーナル |
Int J Syst Evol Microbiol
|
| Abstract |
Two yeast strains isolated from soil collected in Hokkaido, Japan, were found to secrete two extracellular lipases that exhibited activities at both 25 and 4 °C. Both strains could utilize olive oil, rapeseed oil, lard and fish oil as sole carbon sources. The similarity of the D1/D2 domain of the large subunit ribosomal RNA (LSU rRNA) sequence of these yeast strains to that of other yeasts in the GenBank database was very low (<96 %). The phylogenetic trees based on the LSU rRNA sequences and translation elongation factor-1-α (tef1-α) sequences indicated that both strains represented a member of the Wickerhamomyces /Candida clade. Sexual reproduction was not observed. The name Wickerhamomyces psychrolipolyticus f.a., sp. nov is proposed for this newly described yeast species producing cold-active lipases. This novel species is distinguishable from the type strains of other related species, Wickerhamomyces alni, Candida ulmi and Candida quercuum due to their abilities to grow at 4 to 30 °C, to produce lipase that is active also at 4 °C and to assimilate soluble starch.
|
| 巻・号 |
70(2)
|
| ページ |
1158-1165
|
| 公開日 |
2020-2-1
|
| DOI |
10.1099/ijsem.0.003894
|
| PMID |
31833830
|
| MeSH |
Base Composition
DNA, Fungal / genetics
DNA, Ribosomal Spacer / genetics
Japan
Lipase
Mycological Typing Techniques
Peptide Elongation Factor 1 / genetics
Phylogeny*
Ribosome Subunits, Large / genetics
Saccharomycetales / classification*
Saccharomycetales / isolation & purification
Sequence Analysis, DNA
Soil Microbiology*
Temperature
|
| リソース情報 |
| 一般微生物 |
JCM1587
JCM3590
JCM3597
JCM3640 |
