| RRC ID |
66505
|
| Author |
Kajale S, Deshpande N, Pali S, Shouche Y, Sharma A.
|
| Title |
Natrialba swarupiae sp. nov., a halophilic archaeon isolated from a hypersaline lake in India.
|
| Journal |
Int J Syst Evol Microbiol
|
| Abstract |
A haloarchaeal strain (ESP3B_9T) was isolated from the salt pan of Sambhar salt lake, Rajasthan, India. Cells were coccoid, non-motile, Gram-stain-negative and formed reddish-pink pigmented colonies. The strain was aerobic, able to grow at 35-55 °C (optimum, 40 °C), in 20-35 % (25 %) NaCl and pH 8-10 (pH 9). Mg2+ not required for growth. The cells were lysed in distilled water and the minimum NaCl concentration that prevented cell lysis was 5 % w/v. The 16S rRNA gene sequence similarities between strain ESP3B_9T and Natrialba hulunbeirensis JCM 10989T and Natrialba magadii ATCC 43099T were 96.53 and 96.25 % respectively. The similarities of the RNA polymerase subunit B gene between strain ESP3B_9T and N. hulunbeirensis JCM 10989T and N. magadii ATCC 43099T were 84.47 and 84.9 % respectively. Genome sequencing revealed a genome size of 4.20 Mbp with DNA G+C content of 62.5 mol%. The major polar lipids were phosphotidylglycerol and phosphatidylglycerol phosphate methyl esters with minor amounts of unidentified lipids. The results of polyphasic analysis determined that strain ESP3B_9T represents a novel species of the genus Natrialba, for which the name Natrialba swarupiae sp. nov. is proposed. The type strain is ESP3B_9T (MCC 3419T=JCM 33002T=KCTC 4279T=CGMCC 1.16737T).
|
| Volume |
70(3)
|
| Pages |
1876-1881
|
| Published |
2020-3-1
|
| DOI |
10.1099/ijsem.0.003986
|
| PMID |
31967950
|
| MeSH |
Base Composition
DNA, Archaeal / genetics
Halobacteriaceae / classification*
Halobacteriaceae / isolation & purification
India
Lakes / microbiology*
Phospholipids / chemistry
Phylogeny*
Pigmentation
RNA, Ribosomal, 16S / genetics
Salinity
Sequence Analysis, DNA
|
| Resource |
| General Microbes |
JCM33002 |
