RRC ID |
6655
|
著者 |
Saito T, Morio T, Ochiai H.
|
タイトル |
A second functional delta5 fatty acid desaturase in the cellular slime mould Dictyostelium discoideum.
|
ジャーナル |
Eur J Biochem
|
Abstract |
A cDNA with homology to fatty acid desaturases was selected by searching the cDNA data bank of Dictyostelium discoideum (http://www. csm.biol.tsukuba.ac.jp/cDNAproject.html) with conserved histidine box motifs. Using this sequence, genomic DNA encoding the Delta5 desaturase was amplified from the genomic DNA of D. discoideum, and its desaturase activity was confirmed by the overexpression mutation in D. discoideum and the gain-of-function mutation in yeast. The cloned cDNA is 1565 nucleotides in length, and the deduced amino-acid sequence comprised 467 amino-acid residues containing an N-terminal cytochrome b5 domain that shared 43% identity with cytochrome b5 of Oryza sativa. The whole sequence was 42% identical to the Delta5 desaturase of Mortierella alpina. This desaturase is a novel member of the cytochrome b5-containing Delta5 fatty acid desaturase. As we have already reported one other Delta5 desaturase in Dictyostelium, this organism is the first to be confirmed as having two functional Delta5 fatty acid desaturase genes. The substrate specificities of the two functional Delta5 desaturases of D. discoideum were also examined.
|
巻・号 |
267(6)
|
ページ |
1813-8
|
公開日 |
2000-3-1
|
DOI |
10.1046/j.1432-1327.2000.01180.x
|
PII |
ejb1180
|
PMID |
10712614
|
MeSH |
Amino Acid Sequence
Animals
Base Sequence
Caenorhabditis elegans / genetics
Cloning, Molecular
DNA, Complementary / genetics
DNA, Protozoan / genetics
Delta-5 Fatty Acid Desaturase
Dictyostelium / enzymology*
Dictyostelium / genetics
Evolution, Molecular
Fatty Acid Desaturases / genetics
Fatty Acid Desaturases / isolation & purification*
Genes, Protozoan*
Helminth Proteins / chemistry
Isoenzymes / genetics
Isoenzymes / isolation & purification*
Molecular Sequence Data
Oryza / enzymology
Oryza / genetics
Plant Proteins / chemistry
Protein Structure, Tertiary
Protozoan Proteins / genetics
Protozoan Proteins / isolation & purification*
Recombinant Fusion Proteins / chemistry
Saccharomyces cerevisiae
Sequence Homology, Amino Acid
Species Specificity
Substrate Specificity
|
引用数 |
22
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
細胞性粘菌 |
G00774 |