RRC ID 6655
Author Saito T, Morio T, Ochiai H.
Title A second functional delta5 fatty acid desaturase in the cellular slime mould Dictyostelium discoideum.
Journal Eur J Biochem
Abstract A cDNA with homology to fatty acid desaturases was selected by searching the cDNA data bank of Dictyostelium discoideum (http://www. csm.biol.tsukuba.ac.jp/cDNAproject.html) with conserved histidine box motifs. Using this sequence, genomic DNA encoding the Delta5 desaturase was amplified from the genomic DNA of D. discoideum, and its desaturase activity was confirmed by the overexpression mutation in D. discoideum and the gain-of-function mutation in yeast. The cloned cDNA is 1565 nucleotides in length, and the deduced amino-acid sequence comprised 467 amino-acid residues containing an N-terminal cytochrome b5 domain that shared 43% identity with cytochrome b5 of Oryza sativa. The whole sequence was 42% identical to the Delta5 desaturase of Mortierella alpina. This desaturase is a novel member of the cytochrome b5-containing Delta5 fatty acid desaturase. As we have already reported one other Delta5 desaturase in Dictyostelium, this organism is the first to be confirmed as having two functional Delta5 fatty acid desaturase genes. The substrate specificities of the two functional Delta5 desaturases of D. discoideum were also examined.
Volume 267(6)
Pages 1813-8
Published 2000-3-1
DOI 10.1046/j.1432-1327.2000.01180.x
PII ejb1180
PMID 10712614
MeSH Amino Acid Sequence Animals Base Sequence Caenorhabditis elegans / genetics Cloning, Molecular DNA, Complementary / genetics DNA, Protozoan / genetics Delta-5 Fatty Acid Desaturase Dictyostelium / enzymology* Dictyostelium / genetics Evolution, Molecular Fatty Acid Desaturases / genetics Fatty Acid Desaturases / isolation & purification* Genes, Protozoan* Helminth Proteins / chemistry Isoenzymes / genetics Isoenzymes / isolation & purification* Molecular Sequence Data Oryza / enzymology Oryza / genetics Plant Proteins / chemistry Protein Structure, Tertiary Protozoan Proteins / genetics Protozoan Proteins / isolation & purification* Recombinant Fusion Proteins / chemistry Saccharomyces cerevisiae Sequence Homology, Amino Acid Species Specificity Substrate Specificity
Times Cited 22
Cellular slime molds G00774