RRC ID 6663
Author Morio T, Yasukawa H, Urushihara H, Saito T, Ochiai H, Takeuchi I, Maeda M, Tanaka Y.
Title FebA: a gene for eukaryotic translation initiation factor 4E-binding protein (4E-BP) in Dictyostelium discoideum.
Journal Biochim Biophys Acta
Abstract We have identified a gene encoding a eukaryotic initiation factor 4E-binding protein (4E-BP) in the EST database of the Dictyostelium cDNA project. The Dictyostelium 4E-BP, designated febA (four e-binding), showed significant similarity to mammalian 4E-BPs. Northern blot analysis revealed that febA was expressed at a high level in the vegetative growth phase but the level of expression decreased during late development. The gene was shown to be non-essential since disruption of the gene had no severe effect; the null mutant proliferated normally and formed normal fruiting bodies. However, strains overexpressing the gene could not be established, suggesting that an excess of FebA protein may have a lethal effect on the cells.
Volume 1519(1-2)
Pages 65-9
Published 2001-5-28
DOI 10.1016/s0167-4781(01)00219-6
PII S0167-4781(01)00219-6
PMID 11406272
MeSH Amino Acid Sequence Animals Base Sequence Carrier Proteins / genetics* DNA, Complementary / chemistry DNA, Complementary / isolation & purification Dictyostelium / genetics* Genetic Vectors Molecular Sequence Data Phosphoproteins* Sequence Alignment
IF 3.411
Times Cited 0
Cellular slime molds G00593