RRC ID 6667
著者 Daunderer C, Schliwa M, Gräf R.
タイトル Dictyostelium centrin-related protein (DdCrp), the most divergent member of the centrin family, possesses only two EF hands and dissociates from the centrosome during mitosis.
ジャーナル Eur. J. Cell Biol.
Abstract We have identified a Dictyostelium discoideum cDNA sequence with homology to centrins. The derived protein, Dictyostelium discoideum centrinn-related protein (DdCrp), is the most divergent member of the centrin family. Most strikingly it lacks the first two EF-hand consensus motifs, whereas a number of other centrin-specific sequence features are conserved. Southern and Northern blot analysis and the data presently available from the Dictyostelium genome and cDNA projects suggest that DdCrp is the only centrin isoform present in Dictyostelium. Immunofluorescence analysis with anti-DdCrp antibodies revealed that the protein is localized to the centrosome, to a second, centrosome-associated structure close to the nucleus and to the nucleus itself. Confocal microscopy resolved that the centrosomal label is confined to the corona surrounding the centrosome core. Unlike for other centrins the localization of DdCrp is cell cycle-dependent. Both the centrosomal and the centrosome-associated label disappear during prometaphase, most likely in concert with the dissociation of the corona at this stage. The striking differences of DdCrp to all other centrins may be related to the distinct structure and duplication mode of the Dictyostelium centrosome.
巻・号 80(10)
ページ 621-30
公開日 2001-10
DOI 10.1078/0171-9335-00198
PII S0171-9335(04)70179-X
PMID 11713866
MeSH Animals Calcium-Binding Proteins / genetics* Cell Nucleus / metabolism Centrosome / metabolism* Chromosomal Proteins, Non-Histone* Dictyostelium / genetics* Evolution, Molecular Interphase / physiology Mitosis / physiology Molecular Sequence Data Phylogeny Protein Structure, Quaternary Protozoan Proteins / chemistry Protozoan Proteins / genetics* Protozoan Proteins / metabolism* Sequence Homology, Amino Acid
IF 3.712
引用数 22
細胞性粘菌 G02212