| RRC ID |
66677
|
| 著者 |
Hashimoto T, Kozone I, Hashimoto J, Suenaga H, Fujie M, Satoh N, Ikeda H, Shin-Ya K.
|
| タイトル |
Identification, cloning and heterologous expression of biosynthetic gene cluster for desertomycin.
|
| ジャーナル |
J Antibiot (Tokyo)
|
| Abstract |
From our in-house microbial genome database of secondary metabolite producers, we identified a candidate biosynthetic gene cluster for desertomycin from Streptomyces nobilis JCM4274. We report herein the cloning of the 127-kb entire gene cluster for desertomycin biosynthesis using bacterial artificial chromosome vector. The entire biosynthetic gene cluster for desertomycin was introduced in the heterologous host, Streptomyces lividans TK23, with an average yield of more than 130 mg l-1.
|
| 巻・号 |
73(9)
|
| ページ |
650-654
|
| 公開日 |
2020-9-1
|
| DOI |
10.1038/s41429-020-0319-0
|
| PII |
10.1038/s41429-020-0319-0
|
| PMID |
32457441
|
| MeSH |
Chromosomes, Artificial, Bacterial / genetics
Cloning, Molecular / methods
Gene Expression Regulation, Bacterial / genetics*
Genes, Bacterial / genetics*
Macrolides / metabolism*
Multigene Family / genetics*
Streptomyces / genetics
Streptomyces / metabolism
|
| リソース情報 |
| 一般微生物 |
JCM4274 |
