| RRC ID |
66685
|
| 著者 |
Kawai M, Tsuchiya A, Ishida J, Yoda N, Yashiki-Yamasaki S, Katakura Y.
|
| タイトル |
Suppression of lactate production in fed-batch culture of some lactic acid bacteria with sucrose as the carbon source.
|
| ジャーナル |
J Biosci Bioeng
|
| Abstract |
We report a method for suppression of lactate production by lactic acid bacteria (LAB) in culture. LAB produce lactate to regenerate NAD+ that is consumed during glycolysis. Glucose suppresses NAD+ regeneration pathways other than lactate dehydrogenase and non-glycolytic ATP production pathways. Therefore, the carbon source was changed to sucrose, and fed-batch culture was performed to limit the glycolytic flux and thus suppress lactate production. As a result, lactate productivity (i.e., the amount of lactate produced per amount of grown cell) in the sucrose/fed-batch culture was decreased compared to that in glucose/batch culture, in all five LAB strains examined. The productivity level decreased to 24% and 46% in Lactobacillus reuteri JCM 1112 and Lactococcus lactis JCM 7638, respectively. Metabolic flux analysis of Lactobacillus reuteri JCM 1112 revealed increased contributions of the mannitol production pathway to NAD+ regeneration and the arginine deiminase pathway to ATP production in the sucrose/fed-batch culture.
|
| 巻・号 |
129(5)
|
| ページ |
535-540
|
| 公開日 |
2020-5-1
|
| DOI |
10.1016/j.jbiosc.2019.11.009
|
| PII |
S1389-1723(19)30907-7
|
| PMID |
31836379
|
| MeSH |
Bacterial Proteins / metabolism
Batch Cell Culture Techniques
Carbon / metabolism
Fermentation
Glucose / metabolism
Glycolysis
L-Lactate Dehydrogenase / metabolism
Lactic Acid / metabolism*
Lactococcus lactis / growth & development
Lactococcus lactis / metabolism*
Limosilactobacillus reuteri / growth & development
Limosilactobacillus reuteri / metabolism*
NAD / metabolism
Sucrose / metabolism
|
| リソース情報 |
| 一般微生物 |
JCM1112
JCM7638 |
