Reference - Detail
|Author||Aizawa H, Kishi Y, Iida K, Sameshima M, Yahara I.|
|Title||Cofilin-2, a novel type of cofilin, is expressed specifically at aggregation stage of Dictyostelium discoideum development.|
BACKGROUND:A conventional cofilin, cofilin-1 in Dictyostelium discoideum plays significant roles in cell proliferation, phagocytosis, chemotactic movement and macropinocytosis.
RESULTS:We identified a new member of the cofilin family, named cofilin-2 in D. discoideum. Cofilin-2 shows significant homology to a conventional Dictyostelium cofilin, cofilin-1, through its entire sequence, and contains residues conserved among the cofilin family that are responsible for actin-binding. On the other hand, several residues that are conserved among the cofilin family are missing from cofilin-2. Purified cofilin-2 depolymerized actin filaments in a dose- and pH-dependent manner and reduced the apparent viscosity of an actin solution, although they did not co-sediment with actin filaments at all. Cofilin-2 was not expressed in vegetative cells, but was transiently induced during the aggregation stage of development, whereas cofilin-1 was predominantly expressed in vegetative cells. Immunocytochemistry revealed that cofilin-2 localizes at substrate adhesion sites, where cofilin-1 is almost completely excluded. Disruption of the cofilin-2 gene caused an increase in actin accumulation at the substrate adhesion sites. We also found that cofilin-2 did not rescue Deltacof1 yeast cells, whereas cofilin-1 did.
CONCLUSIONS:Cofilin-2 may play a distinct role from that of cofilin-1 in destabilization of the actin cytoskeleton during Dictyostelium development.
|MeSH||Actin Depolymerizing Factors Actins / metabolism Amino Acid Sequence Animals Base Sequence Cell Adhesion Cofilin 2 Cytoskeleton / metabolism Dictyostelium / genetics* Dictyostelium / growth & development* Gene Expression Regulation, Developmental Microfilament Proteins / genetics* Microfilament Proteins / metabolism Molecular Sequence Data Mutation Sequence Homology, Amino Acid Yeasts / genetics|
|WOS Category||GENETICS & HEREDITY CELL BIOLOGY|
|Cellular slime molds||G03271|