論文 - 詳細
| RRC ID | 6676 |
|---|---|
| 著者 | Bogdanovic A, Bennett N, Kieffer S, Louwagie M, Morio T, Garin J, Satre M, Bruckert F. |
| タイトル | Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum. |
| ジャーナル | Biochem J |
| Abstract |
The macropinocytic pathway in Dictyostelium discoideum is organized linearly. After actin-driven internalization, fluid material passes sequentially from endosomes to lysosomes, where molecules are degraded and absorbed. Residual material is exocytosed via post-lysosomal compartments. Syntaxin 7 is a SNARE (soluble N -ethylmaleimide-sensitive fusion protein attachment protein receptor) protein that is present and active in D. discoideum endosomes [Bogdanovic, Bruckert, Morio and Satre (2000) J. Biol. Chem. 275, 36691-36697]. Here we report the identification of its main SNARE partners by co-immunoprecipitation and MS peptide sequencing. The syntaxin 7 complex contains two co-t-SNAREs [Vti1 (Vps10p tail interactor 1) and syntaxin 8] and a v-SNARE [VAMP7 (vesicle-associated membrane protein 7)] (where t-SNAREs are SNAREs of the target compartment and v-SNAREs are SNAREs present in donor vesicles). In endosomes and in vitro, syntaxin 7, Vti1 and syntaxin 8 form a complex that is able to bind VAMP7. Antibodies to syntaxin 8 and a soluble recombinant VAMP7 fragment both inhibit in vitro reconstituted D. discoideum endosome fusion. The lysosomal content of syntaxin 7, Vti1, syntaxin 8 and VAMP7 is low compared with that in endosomes, implying a highly active recycling or retention mechanism. A likely model is that VAMP7 is a v-SNARE present on vesicles carrying lysosomal enzymes, and that the syntaxin 7-Vti1-syntaxin 8 t-SNARE complex is associated with incoming endocytic material. |
| 巻・号 | 368(Pt 1) |
| ページ | 29-39 |
| 公開日 | 2002-11-15 |
| DOI | 10.1042/BJ20020845 |
| PII | BJ20020845 |
| PMID | 12175335 |
| PMC | PMC1222979 |
| MeSH | Amino Acid Sequence Animals Carrier Proteins / metabolism* Dictyostelium / metabolism* Endosomes / metabolism Humans Membrane Fusion Membrane Proteins / metabolism* Molecular Sequence Data Pinocytosis Protozoan Proteins / metabolism* Qa-SNARE Proteins Qb-SNARE Proteins R-SNARE Proteins SNARE Proteins Vesicular Transport Proteins* |
| IF | 4.097 |
| 引用数 | 32 |
| WOS 分野 | BIOCHEMISTRY & MOLECULAR BIOLOGY |
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