RRC ID |
6676
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著者 |
Bogdanovic A, Bennett N, Kieffer S, Louwagie M, Morio T, Garin J, Satre M, Bruckert F.
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タイトル |
Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum.
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ジャーナル |
Biochem J
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Abstract |
The macropinocytic pathway in Dictyostelium discoideum is organized linearly. After actin-driven internalization, fluid material passes sequentially from endosomes to lysosomes, where molecules are degraded and absorbed. Residual material is exocytosed via post-lysosomal compartments. Syntaxin 7 is a SNARE (soluble N -ethylmaleimide-sensitive fusion protein attachment protein receptor) protein that is present and active in D. discoideum endosomes [Bogdanovic, Bruckert, Morio and Satre (2000) J. Biol. Chem. 275, 36691-36697]. Here we report the identification of its main SNARE partners by co-immunoprecipitation and MS peptide sequencing. The syntaxin 7 complex contains two co-t-SNAREs [Vti1 (Vps10p tail interactor 1) and syntaxin 8] and a v-SNARE [VAMP7 (vesicle-associated membrane protein 7)] (where t-SNAREs are SNAREs of the target compartment and v-SNAREs are SNAREs present in donor vesicles). In endosomes and in vitro, syntaxin 7, Vti1 and syntaxin 8 form a complex that is able to bind VAMP7. Antibodies to syntaxin 8 and a soluble recombinant VAMP7 fragment both inhibit in vitro reconstituted D. discoideum endosome fusion. The lysosomal content of syntaxin 7, Vti1, syntaxin 8 and VAMP7 is low compared with that in endosomes, implying a highly active recycling or retention mechanism. A likely model is that VAMP7 is a v-SNARE present on vesicles carrying lysosomal enzymes, and that the syntaxin 7-Vti1-syntaxin 8 t-SNARE complex is associated with incoming endocytic material.
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巻・号 |
368(Pt 1)
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ページ |
29-39
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公開日 |
2002-11-15
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DOI |
10.1042/BJ20020845
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PII |
BJ20020845
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PMID |
12175335
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PMC |
PMC1222979
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MeSH |
Amino Acid Sequence
Animals
Carrier Proteins / metabolism*
Dictyostelium / metabolism*
Endosomes / metabolism
Humans
Membrane Fusion
Membrane Proteins / metabolism*
Molecular Sequence Data
Pinocytosis
Protozoan Proteins / metabolism*
Qa-SNARE Proteins
Qb-SNARE Proteins
R-SNARE Proteins
SNARE Proteins
Vesicular Transport Proteins*
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IF |
4.097
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引用数 |
32
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WOS 分野
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BIOCHEMISTRY & MOLECULAR BIOLOGY
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リソース情報 |
細胞性粘菌 |
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