| RRC ID |
66769
|
| 著者 |
Hatanaka M, Morita H, Aoyagi Y, Sasaki K, Sasaki D, Kondo A, Nakamura T.
|
| タイトル |
Effective bifidogenic growth factors cyclo-Val-Leu and cyclo-Val-Ile produced by Bacillus subtilis C-3102 in the human colonic microbiota model.
|
| ジャーナル |
Sci Rep
|
| Abstract |
Bifidobacterium species are known to fulfill important functions within the human colon. Thus, stimulating the activity of bifidobacteria is important to maintain host health. We revealed that culture supernatants of Bacillus subtilis C-3102 (referred to as C-3102) stimulated the growth of Bifidobacterium species. In this study, we isolated and identified six bifidogenic growth factors, which were cyclo (D-Val-D-Ile), cyclo (L-Val-D-Ile), cyclo (D-Val-L-Ile), cyclo (L-Val-L-Ile), cyclo (D-Val-L-Leu) and cyclo (L-Val-L-Leu). These six cyclic dipeptides increased the growth of Bifidobacterium species and had no effect on potentially harmful gut organisms. Moreover, supplementation with a mixture of these six cyclic dipeptides significantly increased the abundance of microorganisms related to the genus Bifidobacterium in a human colonic microbiota model culture system, although supplementation with a single type of dipeptide had no effect. These results show that cyclic dipeptides containing Val-Leu and Val-Ile produced by C-3102 could serve as bifidogenic growth factors in the gut microbial community.
|
| 巻・号 |
10(1)
|
| ページ |
7591
|
| 公開日 |
2020-5-5
|
| DOI |
10.1038/s41598-020-64374-w
|
| PII |
10.1038/s41598-020-64374-w
|
| PMID |
32372037
|
| PMC |
PMC7200657
|
| MeSH |
Bacillus subtilis / drug effects
Bacillus subtilis / metabolism*
Bifidobacterium / drug effects
Bifidobacterium / growth & development
Chromatography, Liquid
Colon*
Fermentation
Gastrointestinal Microbiome*
Humans
Peptides, Cyclic / analysis
Peptides, Cyclic / biosynthesis*
Peptides, Cyclic / pharmacology
Tandem Mass Spectrometry
|
| リソース情報 |
| 一般微生物 |
JCM1275
JCM1194
JCM1200
JCM1255
JCM1290
JCM1649
JCM11019
JCM5804
JCM1235
JCM20624 |
