論文 - 詳細
| RRC ID | 6678 |
|---|---|
| 著者 | Burlando B, Evangelisti V, Dondero F, Pons G, Camakaris J, Viarengo A. |
| タイトル | Occurrence of Cu-ATPase in Dictyostelium: possible role in resistance to copper. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Dictyostelium discoideum amoebae showed an uncommon resistance to Cu(2+), as pointed out through cell growth rate (EC(50) = 469 +/- 30 microM) and the neutral red cytotoxicity assay (EC(50) = 334 +/- 45 microM). Although no evidence of Cu-inducible metallothionein was found, Cu-dependent ATPase activity was cytochemically detected on pelletted, resin-embedded amoebae. This activity required Cu(2+) in the incubation medium, was sensitive to TPEN, vanadate and temperature, and showed dose-dependent increase after exposure of amoebae to 10-500 microM Cu(2+) for 7 days. Accordingly, immunofluorescence and Western blotting revealed the occurrence of a Cu-inducible, putative homologue of human Menkes (MNK) Cu-P-type ATPase. To verify if Cu-ATPase is involved in copper resistance, amoebae were exposed to low concentrations of Cu(2+) and vanadate followed by the neutral red assay. Exposure to either treatment showed no effect, while a combination caused a dramatic increase of Cu toxicity, possibly depending on Cu-ATPase inhibition. |
| 巻・号 | 291(3) |
| ページ | 476-83 |
| 公開日 | 2002-3-1 |
| DOI | 10.1006/bbrc.2002.6463 |
| PII | S0006291X02964636 |
| PMID | 11855813 |
| MeSH | Adenosine Triphosphatases / analysis Adenosine Triphosphatases / physiology* Animals Blotting, Western Carrier Proteins / analysis Cation Transport Proteins* Cell Division / drug effects Cells, Cultured Copper / pharmacology Copper / toxicity* Dictyostelium / cytology Dictyostelium / drug effects* Dictyostelium / enzymology* Dose-Response Relationship, Drug Drug Resistance Fluorescent Antibody Technique Metallothionein / biosynthesis Microscopy, Confocal Recombinant Fusion Proteins* |
| IF | 2.985 |
| 引用数 | 24 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Policy sources |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | |