Reference - Detail
RRC ID | 6681 |
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Author | Morita T, Amagai A, Maeda Y. |
Title | Unique behavior of a dictyostelium homologue of TRAP-1, coupling with differentiation of D. discoideum cells. |
Journal | Exp Cell Res |
Abstract |
Dd-TRAP1 is a Dictyostelium homologue of TRAP-1, a human protein that binds to the type 1 tumor necrosis factor (TNF) receptor. TRAP-1 has a putative mitochondrial localization sequence and shows significant homology to members of the HSP90 family. Although TRAP-1 is mainly localized to mitochondria in several mammalian cells, in certain tissues it is also localized at specific extramitochondrial sites. In Dictyostelium cells, Dd-TRAP1 is predominantly located in the cell membrane/cortex during growth and just after starvation. Double staining of vegetatively growing cells with the anti-Dd-TRAP1 antibody and TRITC-phalloidin has demonstrated colocalization of Dd-TRAP1 and F-actin at the leading edge of cortical protrusions such as pseudopodes. Coupled with differentiation, however, Dd-TRAP1 located at the cortical region is translocated to mitochondria in spite of the absence of the mitochondrial localization sequence at its N-terminus. The translocation of this protein raises interesting and fundamental questions regarding possible mechanisms by which Dd-TRAP1 is involved in cellular differentiation. |
Volume | 280(1) |
Pages | 45-54 |
Published | 2002-10-15 |
DOI | 10.1006/excr.2002.5620 |
PII | S001448270295620X |
PMID | 12372338 |
MeSH | Actins / metabolism Amino Acid Sequence Animals Cell Differentiation Cell Membrane / metabolism Conserved Sequence Culture Media, Conditioned / metabolism Dictyostelium / chemistry Dictyostelium / growth & development* HSP90 Heat-Shock Proteins / analysis HSP90 Heat-Shock Proteins / chemistry HSP90 Heat-Shock Proteins / genetics HSP90 Heat-Shock Proteins / metabolism* Hydrogen-Ion Concentration Mitochondria / metabolism Molecular Sequence Data Phalloidine / metabolism Protein Structure, Tertiary Protein Transport Recombinant Fusion Proteins / metabolism Rhodamines / metabolism Sequence Alignment Subcellular Fractions / metabolism Transformation, Genetic |
IF | 3.383 |
Times Cited | 22 |
WOS Category | ONCOLOGY CELL BIOLOGY |
Resource | |
Cellular slime molds | G01070 S90287 S90288 |