RRC ID 6684
Author Cornillon S, Dubois A, Brückert F, Lefkir Y, Marchetti A, Benghezal M, De Lozanne A, Letourneur F, Cosson P.
Title Two members of the beige/CHS (BEACH) family are involved at different stages in the organization of the endocytic pathway in Dictyostelium.
Journal J Cell Sci
Abstract Proteins of the Chediak-Higashi/Beige (BEACH) family have been implicated in the function of lysosomes, as well as in signal transduction, but their molecular role is still poorly understood. In Dictyostelium, at least six members of the family can be identified. Here cells with mutations in two of these genes, LVSA and LVSB, were analyzed. Interestingly both mutants exhibited defects in the organization of the endocytic pathway, albeit at distinct stages. In lvsB mutant cells, the regulated secretion of lysosomal enzymes was enhanced, a phenotype reminiscent of the Chediak-Higashi syndrome. LvsA mutant cells exhibited alterations in the organization and function of the early endocytic and phagocytic pathway. The LvsA protein may participate in the signaling pathway, which links adhesion of a particle to the subsequent formation of a phagocytic cup. Further genetic analysis will be necessary to determine whether other members of the BEACH family of proteins are also involved in controlling the organization of the endocytic pathway.
Volume 115(Pt 4)
Pages 737-44
Published 2002-2-15
DOI 10.1242/jcs.115.4.737
PMID 11865029
MeSH Animals Cell Adhesion Dictyostelium / cytology Dictyostelium / genetics Dictyostelium / metabolism* Endocytosis* Kinetics Lysosomes / enzymology Mutation Phagocytosis Phagosomes / enzymology Phagosomes / ultrastructure Protein Structure, Tertiary Proteins / chemistry Proteins / genetics Proteins / physiology* Protozoan Proteins / chemistry Protozoan Proteins / genetics Protozoan Proteins / physiology* Vacuolar Proton-Translocating ATPases / genetics Vacuolar Proton-Translocating ATPases / metabolism
IF 4.573
Times Cited 34
Cellular slime molds G23480 S90284