| RRC ID |
66848
|
| 著者 |
Yamada Y, Nakashima A, Doi S, Ishiuchi N, Kanai R, Miyasako K, Masaki T.
|
| タイトル |
Localization and Maintenance of Engrafted Mesenchymal Stem Cells Administered via Renal Artery in Kidneys with Ischemia-Reperfusion Injury.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
Mesenchymal stem cells (MSCs) are a potential therapeutic tool for preventing the progression of acute kidney injury (AKI) to chronic kidney disease (CKD). Herein, we investigated the localization and maintenance of engrafted human bone marrow-derived MSCs in rats subjected to a renal ischemia-reperfusion injury (IRI) and compared the effectiveness of two intravascular injection routes via the renal artery or inferior vena cava. Renal artery injection of MSCs was more effective than intravenous injection at reducing IRI-induced renal fibrosis. Additionally, MSCs injected through the renal artery persisted in injured kidneys for over 21 days, whereas MSCs injected through the inferior vena cava survived for less than 7 days. This difference may be attributed to the antifibrotic effects of MSCs. Interestingly, MSCs injected through the renal artery were localized primarily in glomeruli until day 3 post-IRI, and they decreased in number thereafter. In contrast, the number of MSCs localized in tubular walls, and the interstitium increased gradually until day 21 post-IRI. This localization change may be related to areas of damage caused by IRI because ischemia-induced AKI leads to tubular cell damage. Taken together, these findings suggest renal artery injection of MSCs may be useful for preventing the progression of AKI to CKD.
|
| 巻・号 |
22(8)
|
| 公開日 |
2021-4-17
|
| DOI |
10.3390/ijms22084178
|
| PII |
ijms22084178
|
| PMID |
33920714
|
| PMC |
PMC8072868
|
| MeSH |
Acute Kidney Injury / therapy*
Animals
Cells, Cultured
Humans
Injections, Intra-Arterial / methods
Male
Mesenchymal Stem Cell Transplantation / methods*
Rats
Rats, Sprague-Dawley
Reperfusion Injury / therapy*
|
| IF |
4.556
|
| リソース情報 |
| ヒト・動物細胞 |
Human mesenchymal stem cells |
