Reference - Detail
| RRC ID | 66878 |
|---|---|
| Author | Kundert P, Sarrion-Perdigones A, Gonzalez Y, Katoh-Kurasawa M, Hirose S, Lehmann P, Venken KJT, Shaulsky G. |
| Title | A GoldenBraid cloning system for synthetic biology in social amoebae. |
| Journal | Nucleic Acids Res |
| Abstract |
GoldenBraid is a rapid, modular, and robust cloning system used to assemble and combine genetic elements. Dictyostelium amoebae represent an intriguing synthetic biological chassis with tractable applications in development, chemotaxis, bacteria-host interactions, and allorecognition. We present GoldenBraid as a synthetic biological framework for Dictyostelium, including a library of 250 DNA parts and assemblies and a proof-of-concept strain that illustrates cAMP-chemotaxis with four fluorescent reporters coded by one plasmid. |
| Volume | 48(8) |
| Pages | 4139-4146 |
| Published | 2020-5-7 |
| DOI | 10.1093/nar/gkaa185 |
| PII | 5813799 |
| PMID | 32232356 |
| PMC | PMC7192589 |
| MeSH | Chemotaxis Cloning, Molecular / methods* Cyclic AMP / physiology Dictyostelium / genetics* Dictyostelium / physiology Luminescent Proteins / genetics Synthetic Biology / methods |
| IF | 11.502 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Cellular slime molds | AX4 |