RRC ID 6690
著者 Sakamoto H, Nishio K, Tomisako M, Kuwayama H, Tanaka Y, Suetake I, Tajima S, Ogihara S, Coukell B, Maeda M.
タイトル Identification and characterization of novel calcium-binding proteins of Dictyostelium and their spatial expression patterns during development.
ジャーナル Dev. Growth Differ.
Abstract Five putative Ca2(+)-binding proteins, CBP5, 6, 7, 8 and 9, all having EF-hand motifs, were found by searching the Dictyostelium cDNA database (http://www.csm.biol.tsukuba.ac.jp/cDNAproject.html). 45Ca2(+)-overlay experiments revealed that four of these (excluding CBP9) are real Ca2(+)-binding proteins. Northern blot analysis revealed that the genes encoding CBP5, 6, 7 and 8 are all developmentally regulated. In situ hybridization analyses revealed that spatial expression of these genes was regulated in several different ways. CBP1, 2, 3, 5, 6 and 7 are expressed in prespore cells in the slug stage. Transcripts of the genes for CBP1 and 5 are enriched in prestalk subtype PstO cells. In contrast, CBP4 is expressed predominantly in PstO cells. CBP8 is evenly expressed at a very low level throughout the whole slug. Such distinct spatial expression patterns suggest that the CBP might be involved in morphogenesis and might have their own roles either in prespore or in prestalk cell differentiation of Dictyostelium.
巻・号 45(5-6)
ページ 507-14
公開日 2004-1-7
PMID 14706075
MeSH Amino Acid Motifs Amino Acid Sequence Animals Blotting, Northern Calcium / chemistry Calcium / metabolism Calcium-Binding Proteins / metabolism* Cell Differentiation Cell Line DNA, Complementary / metabolism Databases as Topic Dictyostelium / cytology* Gene Expression Regulation, Developmental* Glutathione Transferase / metabolism In Situ Hybridization Molecular Sequence Data Phylogeny Recombinant Fusion Proteins / metabolism Sequence Homology, Amino Acid Time Factors
IF 2.145
引用数 10
細胞性粘菌 G01365 G02429 G02123 G02062 G00678