論文 - 詳細
| RRC ID | 6694 |
|---|---|
| 著者 | Watanabe S, Sakurai K, Amagai A, Maeda Y. |
| タイトル | Unexpected roles of a Dictyostelium homologue of eukaryotic EF-2 in growth and differentiation. |
| ジャーナル | J Cell Sci |
| Abstract |
EF-2 is believed to be indispensable for polypeptide chain elongation in protein synthesis and therefore for cell proliferation. Surprisingly, we could isolate ef2 null cells from Dictyostelium discoideum that exhibited almost normal growth and protein synthesis, which suggests that there is another molecule capable of compensating for EF-2 function. The knock-out of Dictyostelium EF-2 (Dd-EF2H; 101 kDa phosphoprotein) impairs cytokinesis, resulting in formation of multinucleate cells. The initiation of differentiation, including the acquisition of aggregation competence, was delayed in Dd-ef2 null cells compared with that in wild-type. By contrast, Dd-ef2 overexpression enhanced the progression of differentiation, thus indicating a positive involvement of Dd-EF2H in growth/differentiation transition. |
| 巻・号 | 116(Pt 13) |
| ページ | 2647-54 |
| 公開日 | 2003-7-1 |
| DOI | 10.1242/jcs.00476 |
| PII | jcs.00476 |
| PMID | 12746492 |
| MeSH | Animals Cell Aggregation / genetics Cell Differentiation / genetics* Cell Division / genetics Cell Nucleus / genetics Cell Nucleus / ultrastructure Dictyostelium / cytology Dictyostelium / metabolism* Giant Cells / cytology Giant Cells / metabolism Mitochondria / genetics Mitochondria / metabolism Molecular Sequence Data Mutation / genetics Peptide Elongation Factor 2 / deficiency* Peptide Elongation Factor 2 / genetics Peptide Elongation Factor 2 / physiology* Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid |
| IF | 4.573 |
| 引用数 | 6 |
| WOS 分野 | CELL BIOLOGY |
| リソース情報 | |
| 細胞性粘菌 | G00508 |