論文 - 詳細
| RRC ID | 6695 |
|---|---|
| 著者 | Kitayama C, Uyeda TQ. |
| タイトル | ForC, a novel type of formin family protein lacking an FH1 domain, is involved in multicellular development in Dictyostelium discoideum. |
| ジャーナル | J Cell Sci |
| Abstract |
Formins are highly conserved regulators of cytoskeletal organization and share three regions of homology: the FH1, FH2 and FH3 domains. Of the nine known formin genes or pseudogenes carried by Dictyostelium, forC is novel in that it lacks an FH1 domain. Mutant Dictyostelium lacking forC (DeltaforC) grew normally during the vegetative phase and, when starved, migrated normally and formed tight aggregates. Subsequently, however, DeltaforC cells made aberrant fruiting bodies with short stalks and sori that remained unlifted. DeltaforC aggregates were also unable to migrate as slugs, suggesting forC is involved in mediating cell movement during multicellular stages of Dictyostelium development. Consistent with this idea, expression of forC was increased significantly in aggregates of wild-type cells. GFP-ForC expressed in DeltaforC cells was localized at the crowns, which are macropinocytotic structures rich in F-actin, suggesting that, like other formin isoforms, ForC functions in close relation with the actin cytoskeleton. Truncation analysis of GFP-ForC revealed that the FH3 domain is required for ForC localization; moreover, localization of a truncated GFP-ForC mutant at the site of contacts between cells on substrates and along the cortex of cells within a multicellular culminant suggests that ForC is involved in the local actin cytoskeletal reorganization mediating cell-cell adhesion. |
| 巻・号 | 116(Pt 4) |
| ページ | 711-23 |
| 公開日 | 2003-2-15 |
| DOI | 10.1242/jcs.00265 |
| PMID | 12538772 |
| MeSH | Actin Cytoskeleton / metabolism* Animals Cell Adhesion / genetics* Cell Aggregation / genetics Cell Compartmentation / genetics Cell Membrane / genetics Cell Membrane / metabolism Dictyostelium / genetics Dictyostelium / metabolism* Fetal Proteins / genetics Fetal Proteins / metabolism Formins Gene Expression Regulation / genetics Microfilament Proteins Molecular Sequence Data Nuclear Proteins / genetics Nuclear Proteins / metabolism Protein Structure, Tertiary / genetics Protozoan Proteins / genetics Protozoan Proteins / isolation & purification* RNA, Messenger / metabolism Recombinant Fusion Proteins Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Up-Regulation / genetics |
| IF | 4.573 |
| 引用数 | 32 |
| WOS 分野 | CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 細胞性粘菌 | G22506 G23048 G23634 S00037 S00038 S00039 S00040 |