論文 - 詳細
| RRC ID | 66963 |
|---|---|
| 著者 | Liu Y, Soh WT, Kishikawa JI, Hirose M, Nakayama EE, Li S, Sasai M, Suzuki T, Tada A, Arakawa A, Matsuoka S, Akamatsu K, Matsuda M, Ono C, Torii S, Kishida K, Jin H, Nakai W, Arase N, Nakagawa A, Matsumoto M, Nakazaki Y, Shindo Y, Kohyama M, Tomii K, Ohmura K, Ohshima S, Okamoto T, Yamamoto M, Nakagami H, Matsuura Y, Nakagawa A, Kato T, Okada M, Standley DM, Shioda T, Arase H. |
| タイトル | An infectivity-enhancing site on the SARS-CoV-2 spike protein targeted by antibodies. |
| ジャーナル | Cell |
| Abstract |
Antibodies against the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein prevent SARS-CoV-2 infection. However, the effects of antibodies against other spike protein domains are largely unknown. Here, we screened a series of anti-spike monoclonal antibodies from coronavirus disease 2019 (COVID-19) patients and found that some of antibodies against the N-terminal domain (NTD) induced the open conformation of RBD and thus enhanced the binding capacity of the spike protein to ACE2 and infectivity of SARS-CoV-2. Mutational analysis revealed that all of the infectivity-enhancing antibodies recognized a specific site on the NTD. Structural analysis demonstrated that all infectivity-enhancing antibodies bound to NTD in a similar manner. The antibodies against this infectivity-enhancing site were detected at high levels in severe patients. Moreover, we identified antibodies against the infectivity-enhancing site in uninfected donors, albeit at a lower frequency. These findings demonstrate that not only neutralizing antibodies but also enhancing antibodies are produced during SARS-CoV-2 infection. |
| 巻・号 | 184(13) |
| ページ | 3452-3466.e18 |
| 公開日 | 2021-6-24 |
| DOI | 10.1016/j.cell.2021.05.032 |
| PII | S0092-8674(21)00662-0 |
| PMID | 34139176 |
| PMC | PMC8142859 |
| MeSH | Animals Antibodies, Monoclonal / immunology* Antibodies, Neutralizing / immunology* Antibodies, Viral / immunology* COVID-19 / immunology Cell Line Chlorocebus aethiops HEK293 Cells Humans Protein Binding / immunology Protein Domains / immunology SARS-CoV-2 / immunology* Spike Glycoprotein, Coronavirus / genetics Spike Glycoprotein, Coronavirus / immunology* Vero Cells |
| IF | 38.637 |
| リソース情報 | |
| ヒト・動物細胞 | 293T(RCB2202) |