| RRC ID |
66966
|
| 著者 |
Takemura Y, Ojima H, Oshima G, Shinoda M, Hasegawa Y, Kitago M, Yagi H, Abe Y, Hori S, Fujii-Nishimura Y, Kubota N, Masuda Y, Hibi T, Sakamoto M, Kitagawa Y.
|
| タイトル |
Gamma-synuclein is a novel prognostic marker that promotes tumor cell migration in biliary tract carcinoma.
|
| ジャーナル |
Cancer Med
|
| Abstract |
Gamma-synuclein (SNCG) promotes invasive behavior and is reportedly a prognostic factor in a range of cancers. However, its role in biliary tract carcinoma (BTC) remains unknown. Consequently, we investigated the clinicopathological significance and function of SNCG in BTC. Using resected BTC specimens from 147 patients with adenocarcinoma (extrahepatic cholangiocarcinoma [ECC, n = 96]; intrahepatic cholangiocarcinoma [ICC, n = 51]), we immunohistochemically evaluated SNCG expression and investigated its correlation with clinicopathological factors and outcomes. Furthermore, cell lines with high SNCG expression were selected from 16 BTC cell lines and these underwent cell proliferation and migration assays by siRNAs. In the results, SNCG expression was present in 22 of 96 (22.9%) ECC patients and in 10 of 51 (19.6%) ICC patients. SNCG expression was significantly correlated with poorly differentiated tumor in both ECC and ICC (p = 0.01 and 0.03, respectively) and with perineural invasion and lymph node metastases in ECC (p = 0.04 and 0.003, respectively). Multivariate analyses revealed that SNCG expression was an independent poor prognostic factor in both OS and RFS in both ECC and ICC. In vitro analyses showed high SNCG expression in three BTC cell lines (NCC-BD1, NCC-BD3, and NCC-CC6-1). Functional analysis revealed that SNCG silencing could suppress cell migration in NCC-BD1 and NCC-CC6-1 and downregulate cell proliferation in NCC-CC6-1 significantly. In conclusion, SNCG may promote tumor cell activity and is potentially a novel prognostic marker in BTC.
|
| 巻・号 |
10(16)
|
| ページ |
5599-5613
|
| 公開日 |
2021-8-1
|
| DOI |
10.1002/cam4.4121
|
| PMID |
34245137
|
| PMC |
PMC8366101
|
| MeSH |
Aged
Aged, 80 and over
Bile Duct Neoplasms / mortality*
Bile Duct Neoplasms / pathology
Bile Duct Neoplasms / surgery
Bile Ducts, Extrahepatic / pathology
Bile Ducts, Extrahepatic / surgery
Bile Ducts, Intrahepatic / pathology
Bile Ducts, Intrahepatic / surgery
Biomarkers, Tumor / analysis
Biomarkers, Tumor / genetics
Biomarkers, Tumor / metabolism*
Cell Line, Tumor
Cell Movement
Cholangiocarcinoma / mortality*
Cholangiocarcinoma / pathology
Cholangiocarcinoma / surgery
Disease-Free Survival
Female
Follow-Up Studies
Gene Knockdown Techniques
Humans
Male
Middle Aged
Neoplasm Proteins / analysis
Neoplasm Proteins / genetics
Neoplasm Proteins / metabolism*
Prognosis
gamma-Synuclein / analysis
gamma-Synuclein / genetics
gamma-Synuclein / metabolism*
|
| IF |
3.491
|
| リソース情報 |
| ヒト・動物細胞 |
TKKK(RCB1907)
HuCCT1(RCB1960) |
